Analysis of proteins in the extracts of Physalis alkekengi L. var. franchetii (Mast.) Makino using nanoflow reversed-phase liquid chromatography-tandem mass spectrometry

doi:10.3724/SP.J.1123.2012.11023

Abstract

Abstract: The protein was extracted from Physalis alkekengi L. var. franchetii (Mast.) Makino fruit by using water extraction and acid precipitation methods, and it consisted of 188 mg/g of protein on dry basis of the extract. Among 18 amino acids, eight essential amino acids for human account for 31% were found in this extract. Based on shotgun proteomics method, the protein extracted from Physalis fruit was analysed by nanoflow reversed-phase liquid chromatography-tandem mass spectrometry (nano-RPLC-MS/MS) system. Combined with database searches and bioinformatics analysis, the protein species and six molecular functions were identified, including with catalytic activity, antioxidant activity, enzyme regular activity, nutrient reservoir activity, transporter activity and binding activity. And three antioxidant activity-related proteins were identified. These results may lay the foundation for further study of the functional properties of the proteins in Physalis fruit.

Key words: Physalis alkekengi L. var. franchetii (Mast.) Makino, nanoflow reversed-phase liquid chromatography-tandem mass spectrometry (nano-RPLC-MS/MS), protein

CLC Number:

O658

YU Haiyang, YAN Jiaze, GUO Ming, JIN Yan^*. Analysis of proteins in the extracts of Physalis alkekengi L. var. franchetii (Mast.) Makino using nanoflow reversed-phase liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2013, 31(4): 362-366.

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