Chinese Journal of Chromatography ›› 2016, Vol. 34 ›› Issue (3): 232-239.DOI: 10.3724/SP.J.1123.2015.10030

• Articles • Previous Articles     Next Articles

Preparation and properties of new nickel ion affinity chromatography medium

XU Lili1, CHEN Geyu1, ZHANG Teng1, ZHANG Zhongqi2, HAN Guang2, LI Qian2, ZHAO Jinli2, LIU Jianli1   

  1. 1. Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University, Xi'an 710069, China;
    2. Active Protein & Polypeptide Engineering Center of Xi'an Hua Ao Li Kang, Xi'an 710054, China
  • Received:2015-10-20 Online:2016-03-08 Published:2012-09-28
  • Supported by:

    National Natural Science Foundation of China (Nos. 30070905, 20872118); Foundation of Key Laboratory of Shaanxi Province (No. 2010JS097); Foundation of Shaanxi Provincial Department of Education (No. 08jk477).

Abstract:

With the chloromethylated crosslinking polystyrene microspheres (chlorine ball) as the matrices, N,N-dimethylformamide (DMF) as the solvent, activated by N,N-diisopropylethylamine (DIEA), the synthesized Lys(Boc)-OEt and ethyl chloroacetate were coupled to the matrices. After hydrolysis, the carboxyl group was released and chelated with Ni2+, and a new nickel ion affinity chromatographic medium was prepared. The affinity medium was applied to purify the His-Lys-Tyr and Phe-His-Thr tri-peptides. The results showed that the peptides containing histidine were retained on the affinity medium and separated with other substances.

Key words: chloromethylated crosslinking polystyrene microspheres, histidine-containing peptides, nickel ion affinity chromatography medium

CLC Number: