Rapid screening of acidity regulators in dairy by ion chromatography-high resolution mass spectrometry

doi:10.3724/SP.J.1123.2017.04012

Abstract

Abstract:

A method for screening of acidity regulators in dairy based on ion chromatography-high resolution mass spectrometry technology (IC-HRMS) was set up. The dairy samples were extracted by KOH (pH 7-8) and Oasis MAX SPE column, and separated by a Dionex IonPac AS11-HC column (250 mm×4 mm). All the acidity regulators were detected by Orbitrap full scan mode. Taking six organic acids as an example, the calibration curves showed good linearities in the range of 0.05-5.00 mg/L, and the correlation coefficients (r) were higher than 0.99. By detecting the spiked samples, the recoveries were in the range of 74.3%-115.5% with the relative standard deviations (RSDs) between 0.64% and 4.81%. Malic acid, citric acid, lactic acid, succinic acid and adipic acid could be detected by IC-HRMS in the commercial dairy samples. The results indicate that the method is simple, rapid and suitable for the qualitative screening of acidity regulators in dairy products.

Key words: acidity regulators, dairy, ion chromatography-high resolution mass spectrometry (IC-HRMS), rapid screening

CLC Number:

O658

YUN Huan, LIU Xin, CUI Jie, YANG Jing, LIU Ying. Rapid screening of acidity regulators in dairy by ion chromatography-high resolution mass spectrometry[J]. Chinese Journal of Chromatography, 2017, 35(8): 886-890.

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