Separation of chemical compositions in root of Rumex patientia L. with off-line two-dimensional liquid chromatography

doi:10.3724/SP.J.1123.2017.09021

Chinese Journal of Chromatography ›› 2018, Vol. 36 ›› Issue (1): 37-42.DOI: 10.3724/SP.J.1123.2017.09021

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Separation of chemical compositions in root of Rumex patientia L. with off-line two-dimensional liquid chromatography

ZHAO Yaqing¹, FU Dongmei², LIU Yanfang², LIANG Xinmiao², XUE Huiqing¹

1. Shanxi Key Laboratory of Innovative Drug for the Treatment of Serious Diseases Basing on the Chronic Inflammation, Shanxi University of Traditional Chinese Medicine, Taiyuan 030001, China;
2. Key Laboratory of Separation and Analysis Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116021, China

Received:2017-09-12 Online:2018-01-08 Published:2018-01-22
Supported by:
Scientific and Technological Project in Shanxi Province (No. 2014ZD0101); Special Beijing Science and Technology Commission of Technology-based Smes (No. Z17010101216).

Abstract

Abstract:

An innovative analytical method based on the off-line two-dimensional reversed-phase/reversed-phase liquid chromatography (2D-RPLC/RPLC) was established to separate the components of root of Rumex patientia L. The chromatograms of ethyl acetate extract of root of Rumex patientia L. on a phenyl/tetrazolium column (250 mm×4.6 mm, 7 μm) and a Unitary C18 column (250 mm×4.6 mm, 7 μm) were compared, and they showed different separation abilities. A phenyl/tetrazolium column was used for the first dimensional separation with 0.1% (v/v) formic acid aqueous solution and methanol as mobile phases, and 18 fractions was collected. The second dimensional liquid chromatography analysis was carried out on a Unitary C18 column with 0.1% (v/v) formic acid aqueous solution and methanol as mobile phases. Based on the experiment setup and results, it was concluded that off-line 2D-LC can be an effective method for the separation of the trace components and the screening of active compounds of root of Rumex patientia L.

Key words: Rumex patientia L., anthraquinone, phenyl/tetrazolium bonded stationary phase, two-dimensional liquid chromatography (2D-LC)

CLC Number:

O658

ZHAO Yaqing, FU Dongmei, LIU Yanfang, LIANG Xinmiao, XUE Huiqing. Separation of chemical compositions in root of Rumex patientia L. with off-line two-dimensional liquid chromatography[J]. Chinese Journal of Chromatography, 2018, 36(1): 37-42.

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Separation of chemical compositions in root of Rumex patientia L. with off-line two-dimensional liquid chromatography

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