Chinese Journal of Chromatography

Chinese Journal of Chromatography

• 研究简报 • Previous Articles     Next Articles

Preparative Isolation and Purification of Calycosin and Formononetin from Astragalus membranaceus

MA Xiaofeng1,2, TU Pengfei1,CHEN Yingjie 2, ZHANG Tianyou3, WEI Yun3   

  1. 1. Modern Research Center for Traditional Chinese Medicine, Peking University, Beijing 100083, China;2. Shenyang Pharmaceutical University, Shenyang 110016, China;3. Beijing Institute of New Technology Application, Beijing 100035, China
  • Received:2004-06-15 Revised:1900-01-01 Online:2005-05-30 Published:1988-09-25

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Abstract: Preparative high-speed counter-current chromatography (HSCCC) was used for the separation of calycosin and formononetin from the ethyl acetate extract of Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao. Calycosin was obtained by HSCCC with a binary phase solvent system composed of n-hexane-chloroform-methanol-water (1.5∶3∶3∶2, v/v). Then, formononetin was obtained with a system composed of n-hexane-chloroform-methanol-water (4∶4∶5∶4, v/v). The mobile phase was the lower phase and operated at a flow-rate of 2.0 mL/min, while the apparatus rotated at 800 r/min. High performance liquid chromatographic analysis of calycosin and formononetin revealed that their purities were over 95%. HSCCC is a useful method for the separation of natural products.

Key words: Astragalus , calycosin, formononetin, high-speed counter-current chromatography