Chinese Journal of Chromatography

Chinese Journal of Chromatography

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Comparison of Supercritical Fluid Extraction and Steam Distillation Methods for the Extraction of Essential Oils from Schizonepeta tenuifolia Briq.

QIU Qin1, LING Jianya2, DING Yuping1, CHANG Hongwen1, WANG Jiang1, LIU Tingli1   

  1. 1. School of Environmental Science and Engineering, Shandong University, Jinan 250100, China;
    2. State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China
  • Received:2004-10-28 Revised:1900-01-01 Online:2005-11-30 Published:1987-12-25

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Abstract:

Essential oil was extracted from Schizonepeta tenuifolia Briq. by supercritical fluid extraction (SFE) and steam distillation (SD). The components extracted were determined by gas chromatography with area normalization method and identified by gas chromatography-mass spectrometry (GC-MS). The optimal chromatographic conditions were: capillary column, SE-54 (30 m×0.25 mm i.d., 0.25 μm); column temperature, 50 ℃(3 min)5 ℃/min180 ℃(2 min)10 ℃/min260 ℃(50 min); split injection, split ratio 1∶50; injector temperature, 280 ℃. Fifty-four components were identified for the essential oils extracted by SFE, and its main components were found to be pulegone, menthone, linoleic acid chloride etc. Thirty-nine components were identified for the essential oil obtained by SD, and its main components were found to be pulegone, menthone, limonene etc. The SFE method is better than the SD method in reliability stability and reproducibility, and is thus well suitable for similar applications involving for extraction of other traditional Chinese herbal medicines.

Key words: gas chromatography-mass spectrometry, Schizonepeta tenuifolia Briq., steam distillation, traditional Chinese herbal medicine , supercritical fluid extraction