Chinese Journal of Chromatography

Chinese Journal of Chromatography

• 研究简报 • Previous Articles     Next Articles

Separation of Proteins by Gradient Pressurized Capillary Electrochromatography

ZHAO Liangjuan1, ZOU Juanjuan3, WANG Xiuli3, YAN Chao3, GAO Ruyu2, LIU Mingyu4, Lv Xianyu1   

  1. 1. College of Life Science, Nankai University, Tianjin 300071, China; 2. State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin 300071, China; 3. Unimicro (Shanghai) Technologies
    Co., Ltd., Shanghai 201203, China; 4. China Agricultural University, Beijing 100094, China
  • Received:2004-12-21 Revised:1900-01-01 Online:2005-11-30 Published:1987-12-25

PDF

756

Abstract:

The separation of four proteins including RNase A, cytochrome C, lysozyme and myoglobin was investigated by reversed-phase gradient pressurized capillary electrochromatography (p-CEC) with 1.5 μm non-porous silica C18 stationary phase. This mode was compared with micro-high performance liquid chromatography (μ-HPLC) and the effects of applied voltage, stationary phase and concentration of ion-pairing agent (trifluoroacetic acid, TFA) on the gradient p-CEC were also studied. This separation was performed rapidly on a new CEC instrument TrisepTM 2010 GV. The results showed that the retention mechanism of proteins in p-CEC mode is based on both chromatographic partitioning and electrophoretic migration. The results also demonstrated that p-CEC may have great potential for fast and efficient separation of proteins.

Key words: pressurized capillary electrochromatography, proteins , reversed-phase chromatography, gradient elution