色谱

色谱

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高效液相色谱-荧光检测法测定罂粟籽和火锅汤料中的罂粟碱

皮立1,2,胡凤祖1,师治贤1

  

  1. 1. Northwest Plateau Institute of Biology, The Chinese Academy of Sciences, Xining 810001, China;2. Graduate School of The Chinese Academy of Sciences, Beijing 100049, China
  • 收稿日期:2004-11-29 修回日期:1900-01-01 出版日期:2005-11-30 发布日期:1987-12-25
  • 通讯作者: 胡凤祖

Determination of Papaverine in Seeds of Papaver somniferum L.and Soup of Chafing Dish by High Performance Liquid Chromatography-Fluorescence Detection

PI Li1,2, HU Fengzu1, SHI Zhixian1   

  1. 1. Northwest Plateau Institute of Biology, The Chinese Academy of Sciences, Xining 810001, China;2. Graduate School of The Chinese Academy of Sciences, Beijing 100049, China
  • Received:2004-11-29 Revised:1900-01-01 Online:2005-11-30 Published:1987-12-25

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摘要：

用所建立的高效液相色谱-荧光检测法测定了罂粟籽和火锅汤料中的罂粟碱。采用的色谱柱为RP-C18柱(250 mm×4.6 mm i.d.,5 μm);检测激发波长为285 nm,发射波长为355 nm;流动相为甲醇-0.02 mol/L乙酸铵(体积比为70∶30),流速0.8 mL/min。实验结果表明,罂粟碱的进样量为1×10-4~0.1 μg时其质量浓度与相应峰面积有良好的线性关系,最低检测限(以信噪比大于3计)达到0.02 ng。罂粟籽中罂粟碱的回收率为99.0%～100.8%。方法快速准确,简便灵敏,分离度高,能够满足有关食品中罂粟碱的检测要求。

关键词: 高效液相色谱法, 火锅汤 , 罂粟碱, 罂粟籽, 荧光检测

Abstract:

A method of reversed-phase high performance liquid chromatography coupled with fluorescence detection was developed for the separation and determination of papaverine in seeds of Papaver somniferum L. and soup of chafing dish. The liquid chromatographic experiment was carried out under the following conditions: the detector was a fluorescence with excitation wavelength at 285 nm and emission wavelength at 355 nm. An RP-C18 column (250 mm×4.6 mm i.d., 5 μm) was used. The mobile phase was a mixture of methanol and 0.02 mol/L ammonium acetate (70∶30, v/v). The flow rate was 0.8 mL/min. A linear range was obtained from 0.1 ng to 0.1 μg with a good correlation. The lowest detection limit was 0.02 ng. The average recovery of seeds for Papaver somniferum L. was 99.0%-100.8%. The method is rapid, accurate, sensitive and suitable for the separation and determination of papaverine in foods.

Key words: fluorescence detection, papaverine, seed of Papaver somniferum L, soup of chafing dish , high performance liquid chromatography