Chinese Journal of Chromatography ›› 2021, Vol. 39 ›› Issue (6): 642-651.DOI: 10.3724/SP.J.1123.2020.08024
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XU Xia, LI Ting, JIA Jinru, TANG Huiting, LI Jun, ZHAO Yunfang*(), SONG Yuelin*()
Received:
2020-08-22
Online:
2021-06-08
Published:
2021-04-13
Contact:
ZHAO Yunfang,SONG Yuelin
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CLC Number:
XU Xia, LI Ting, JIA Jinru, TANG Huiting, LI Jun, ZHAO Yunfang, SONG Yuelin. Analysis of chemical components of Chinese medicine Ligustici Radix by achiral-chiral liquid chromatography-predictive multiple reaction monitoring[J]. Chinese Journal of Chromatography, 2021, 39(6): 642-651.
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URL: https://www.chrom-china.com/EN/10.3724/SP.J.1123.2020.08024
No. | tR/ min | Molecular formula | Fragment ions (m/z) | Precursor ion (m/z) | Product ion (m/z) | Putative identity | DP/ V | CE/ eV | Chemical class | Ref. |
---|---|---|---|---|---|---|---|---|---|---|
1 | 2.92 | C5H9NO4 | 129.60; 106.32; 84.04 | 148.06 | 84.04 | glutamic acid | 25 | 23 | AA | [ |
2 | 3.00 | C10H13N5O5 | 152.06 | 284.09 | 152.06 | guanosine | 50 | 25 | AA | [ |
3 | 3.15 | C6H13NO2 | 113.08; 86.08 | 132.10 | 86.08 | isoleucine | 50 | 18 | AA | [ |
4 | 3.25 | C6H13NO2 | 113.08; 86.08 | 132.10 | 86.08 | leucine | 40 | 30 | AA | [ |
5 | 5.09 | C15H16O8 | 163.04; 53.78 | 325.09 | 163.04 | skimmin | 80 | 25 | SC | [ |
6 | 12.61 | C14H14O5 | 245.08; 227.07; 191.03 | 263.09 | 245.08 | trans-khellactone (TKL) | 80 | 24 | AP | [ |
7 | 13.89 | C14H14O5 | 245.08; 191.04* | 263.09 | 245.08 | (3'S)-cis-khellactone (D-CKL) | 80 | 27 | AP | [ |
8 | 14.16 | C14H14O5 | 245.08; 191.04* | 263.09 | 245.08 | (3'R)-cis-khellactone (L-CKL) | 80 | 27 | AP | [ |
9 | 15.84 | C20H24O9 | 247.09; 229.08; 175.04 | 409.15 | 247.09 | nodakenin | 80 | 23 | LF | [ |
10 | 19.50 | C14H14O5 | 245.16*; 227.04; 203.06; 175.04 | 263.09 | 203.06 | (D/L)-qianhucoumarin G (QC-G) | 80 | 20 | LF | [ |
11 | 19.70 | C14H14O5 | 245.16*; 227.04; 203.06; 175.04 | 263.09 | 203.06 | (D/L)-qianhucoumarin G | 80 | 20 | LF | [ |
12 | 22.31 | C13H10O5 | 189.03 | 264.08 | 189.03 | isopimpinellin | 80 | 25 | LF | [ |
13 | 23.91 | C21H24O9 | 361.13; 245.08 | 438.17 | 245.08 | 3'-(hydroxyl-isovaleryl/2-methylbutyroyl)- 4'-hydroxyl-acetylkhellactone | 80 | 30 | AP | [ |
14 | 24.20 | C21H24O9 | 361.14; 245.08; 187.03 | 438.17 | 245.08 | 3'-(hydroxyl-isovaleryl/2-methylbutyroyl)-4'-hydroxyl-acetylkhellactone | 80 | 30 | AP | [ |
15 | 24.22 | C16H16O6 | 287.10; 245.08 | 305.10 | 245.08 | qianhucoumarin B | 80 | 30 | AP | [ |
16 | 24.86 | C21H24O9 | 361.14; 245.08; 187.03 | 438.17 | 245.08 | 3'-(hydroxyl-isovaleryl/2-methylbutyroyl)-4'-hydroxyl-acetylkhellactone | 80 | 30 | AP | [ |
17 | 25.73 | C12H8O4 | 202.03; 174.06 | 217.04 | 202.03 | bergapten or methoxsalen | 80 | 20 | LF | [ |
18 | 27.17 | C21H22O8 | 343.12; 245.08 | 420.16 | 343.12 | 3'-(hydroxyl-tigloyl/senecioyl/angeloyl)- 4'-acetylkhellactone | 80 | 15 | AP | [ |
19 | 27.57 | C21H22O8 | 343.11; 287.09; 245.08 | 420.16 | 343.11 | 3'-(hydroxyl-tigloyl/angeloyl/senecioyl)- 4'-acetylkhellactone | 80 | 15 | AP | [ |
20 | 29.29 | C16H14O5 | 245.08; 217.08; 175.04 | 287.09 | 245.08 | 3'-acetyloxy-3',4'-dihydroseselin or 4'- acetyloxy-3',4'-dihydroseselin | 80 | 35 | AP | [ |
21 | 29.31 | C18H18O7 | 287.09; 245.08; 227.07 | 369.09 | 245.08 | qianhucoumarin D | 80 | 32 | AP | [ |
22 | 29.85 | C12H8O4 | 202.03; 174.06; 165.72 | 217.04 | 202.03 | bergapten or methoxsalen | 80 | 20 | LF | [ |
23 | 30.70 | C22H26O7 | 329.14; 315.12; 245.08; 227.08 | 420.20 | 343.12 | 3'-(hydroxyl-tigloyl/angeloyl/senecioyl)- 4'-acetylkhellactone | 80 | 15 | AP | [ |
24 | 31.00 | C22H26O7 | 329.14; 315.12; 245.08; 227.07 | 420.20 | 343.12 | 3'-(hydroxyl-tigloyl/angeloyl/senecioyl)- 4'-acetylkhellactone | 80 | 15 | AP | [ |
25 | 31.40 | C22H26O7 | 329.14; 315.12; 245.08; 227.07 | 420.20 | 329.14 | 3'-(isovaleryl/2-methylbutyroyl)-4'-propi- onylkhellactone | 80 | 30 | AP | [ |
26 | 32.15 | C19H20O7 | 287.09; 245.08 | 378.15 | 245.08 | 3'-acetyl-4'-propionylkhellactone | 80 | 30 | AP | [ |
27 | 32.34 | C19H20O7 | 287.09; 245.08 | 378.15 | 245.08 | 3'-acetyl-4'-propionylkhellactone isomer | 80 | 30 | AP | [ |
28 | 33.52 | C19H20O6 | 245.08; 217.07; 205.04; 191.04 | 345.13 | 245.08 | Pd-C-I | 80 | 30 | LP | [ |
29 | 35.18 | C20H22O7 | 287.09; 245.08 | 392.17 | 245.08 | bocconin | 80 | 15 | AP | [ |
30 | 35.61 | C19H20O6 | 345.13; 245.08; 261.05 | 362.15 | 261.05 | 3'-(isovaleryloxy/2-methylbutyroyloxy)- 4'-oxo-3',4'-dihydroseselin | 80 | 30 | AP | [ |
31 | 35.64 | C20H22O7 | 287.09; 245.08 | 392.17 | 245.08 | isobocconin | 80 | 15 | AP | [ |
32 | 36.19 | C19H20O6 | 345.13; 261.06; 245.08 | 362.15 | 261.06 | 3'-(isovaleryloxy/2-methylbutyroyloxy)- 4'-oxo-3',4'-dihydroseselin | 80 | 30 | AP | [ |
33 | 36.27 | C11H6O4 | 175.04; 147.05 | 203.03 | 147.05 | xanthotoxol | 80 | 40 | LF | [ |
34 | 36.45 | C16H14O4 | 203.03; 175.04; 159.03; 147.04; 131.02 | 271.09 | 203.03 | imperatorin | 80 | 18 | LF | [ |
35 | 37.69 | C21H22O7 | 327.12; 287.09; 245.08; 227.07* | 409.12 | 245.08 | (3'S)-pteryxin (D-Pte) | 80 | 20 | AP | [ |
36 | 37.71 | C16H14O5 | 245.08; 217.09; 175.04 | 287.09 | 245.08 | 4'-acetyl-khellactone | 80 | 32 | AP | [ |
37 | 38.04 | C21H22O7 | 327.12; 287.09; 245.08*; 227.07 | 409.12 | 227.07 | (3'S)-praeruptorin A (D-PA) | 80 | 19 | AP | [3,22] |
38 | 38.12 | C24H28O7 | 349.10; 327.12; 251.06; 245.08; 227.07 | 451.17 | 245.08 | 3'-(isovaleryl/2-methylbutyroyl)-4'-(tigloyl/ senecioyl/angeloyl)-khellactone | 80 | 34 | AP | [ |
39 | 38.50 | C17H16O5 | 283.08; 233.04; 218.05; 189.12; 173.03 | 301.10 | 233.04 | cnidilin | 80 | 37 | LF | [ |
40 | 38.94 | C16H14O5 | 245.08; 175.04 | 287.09 | 245.08 | 3'-acety-4'-dehydro-decusinol | 80 | 32 | LP | - |
41 | 39.01 | C23H28O7 | 351.12; 245.08; 227.07 | 434.21 | 245.08 | 3'-(2-isobutyroyl)-4'-(isovaleryl/2-meth- ylbutyroyl)-khellactone | 80 | 30 | AP | [ |
42 | 39.03 | C21H24O7 | 329.13*; 287.09; 245.08 | 406.18 | 245.08 | (3'S)-3'-isovaleryl-4'-acetylkhellactone (D-IAK) | 80 | 30 | AP | [ |
43 | 39.49 | C21H22O7 | 327.12; 287.09; 245.07; 227.07* | 409.12 | 245.08 | (3'R)-pteryxin (L-Pte) | 80 | 20 | AP | [ |
44 | 39.58 | C21H22O7 | 327.12; 287.09; 245.08*; 227.07 | 409.12 | 227.07 | (3'R)-praeruptorin A (L-PA) | 80 | 19 | AP | [ |
45 | 41.42 | C16H14O4 | 203.05; 175.04; 159.03; 147.03; 131.02 | 271.06 | 203.05 | isoimperatorin | 80 | 18 | LF | [ |
46 | 41.60 | C21H24O7 | 329.13*; 287.09; 245.08 | 406.18 | 245.08 | (3'R)-3'-isovaleryl-4'-acetylkhellactone (L-IAK) | 80 | 30 | AP | [ |
47 | 45.84 | C23H26O7 | 315.12; 245.08 | 432.20 | 315.12 | 3'-(2-isobutyroyl)-4'-(tigloyl/senecioyl/angeloyl)-khellactone | 80 | 15 | AP | [ |
48 | 46.05 | C23H26O7 | 327.13; 315.12; 227.09 | 432.20 | 227.09 | 3'-(2-isobutyroyl)-4'-(tigloyl/senecioyl/ angeloyl)-khellactone | 80 | 30 | AP | [ |
49 | 47.41 | C24H26O7 | 327.12; 227.07 | 444.20 | 327.12 | 3'-(tigloyl/senecioyl/angeloyl)-4'-(tigloyl/ senecioyl/angeloyl)-khellactone | 80 | 25 | AP | [3,4 |
50 | 48.16 | C24H26O7 | 327.12; 245.08; 217.08; 189.02 | 444.20 | 327.12 | 3'-(tigloyl/senecioyl/angeloyl)-4'-(tigloyl/ senecioyl/angeloyl)-khellactone | 80 | 26 | AP | [ |
51 | 48.90 | C23H28O7 | 351.13; 245.08; 227.07 | 434.21 | 245.08 | 3'-(2-isobutyroyl)-4'-(isovaleryl/2-meth- ylbutyroyl)-khellactone | 80 | 30 | AP | [ |
52 | 49.65 | C24H26O7 | 349.11; 327.12; 245.08*; 227.06 | 444.20 | 327.12 | (3'S)-praeruptorin B(D-PB) | 80 | 36 | AP | [ |
53 | 50.28 | C24H28O7 | 329.14; 327.12; 227.06; | 451.17 | 245.08 | 3'-(isovaleryl/2-methylbutyroyl)-4'-(tigloyl/ senecioyl/angeloyl)-khellactone | 80 | 34 | AP | [ |
54 | 50.50 | C24H26O7 | 349.11; 327.12; 245.08*; 227.06 | 444.20 | 327.12 | (3'R)-praeruptorin B (L-PB) | 80 | 36 | AP | [ |
55 | 51.61 | C24H28O7 | 349.11; 327.11; 251.06; 245.08; 227.07* | 451.17 | 245.08 | (3'S)-praeruptorin E (D-PE) | 80 | 35 | AP | [ |
56 | 54.31 | C24H28O7 | 349.11; 327.11; 251.06; 245.08; 227.07* | 451.17 | 245.08 | (3'R)-praeruptorin E (L-PE) | 80 | 35 | AP | [ |
57 | 55.08 | C24H30O7 | 329.13; 245.08*; 227.06 | 448.23 | 227.06 | (3'S)-3',4'-diisovalerylkhellactone (D-DIK) | 80 | 55 | AP | [ |
58 | 56.49 | C24H30O7 | 329.13; 245.08*; 227.06 | 448.23 | 227.06 | (3'R)-3',4'-diisovalerylkhellactone (L-DIK) | 80 | 55 | AP | [ |
59 | 63.18 | C24H30O7 | 329.13; 245.08; 227.06 | 448.23 | 227.06 | 3',4'-diisovalerylkhellactone isomer | 80 | 55 | AP | |
60 | 64.75 | C24H26O7 | 327.12; 245.08; 217.08; 189.02 | 444.20 | 327.12 | 3'-(tigloyl/senecioyl/angeloyl)-4'-(tigloyl/ senecioyl/angeloyl)-khellactone | 80 | 36 | AP | [ |
Table 1 MS information of components in Chinese medicine Ligustici Radix using achiral-chiral LC-predictive MRM
No. | tR/ min | Molecular formula | Fragment ions (m/z) | Precursor ion (m/z) | Product ion (m/z) | Putative identity | DP/ V | CE/ eV | Chemical class | Ref. |
---|---|---|---|---|---|---|---|---|---|---|
1 | 2.92 | C5H9NO4 | 129.60; 106.32; 84.04 | 148.06 | 84.04 | glutamic acid | 25 | 23 | AA | [ |
2 | 3.00 | C10H13N5O5 | 152.06 | 284.09 | 152.06 | guanosine | 50 | 25 | AA | [ |
3 | 3.15 | C6H13NO2 | 113.08; 86.08 | 132.10 | 86.08 | isoleucine | 50 | 18 | AA | [ |
4 | 3.25 | C6H13NO2 | 113.08; 86.08 | 132.10 | 86.08 | leucine | 40 | 30 | AA | [ |
5 | 5.09 | C15H16O8 | 163.04; 53.78 | 325.09 | 163.04 | skimmin | 80 | 25 | SC | [ |
6 | 12.61 | C14H14O5 | 245.08; 227.07; 191.03 | 263.09 | 245.08 | trans-khellactone (TKL) | 80 | 24 | AP | [ |
7 | 13.89 | C14H14O5 | 245.08; 191.04* | 263.09 | 245.08 | (3'S)-cis-khellactone (D-CKL) | 80 | 27 | AP | [ |
8 | 14.16 | C14H14O5 | 245.08; 191.04* | 263.09 | 245.08 | (3'R)-cis-khellactone (L-CKL) | 80 | 27 | AP | [ |
9 | 15.84 | C20H24O9 | 247.09; 229.08; 175.04 | 409.15 | 247.09 | nodakenin | 80 | 23 | LF | [ |
10 | 19.50 | C14H14O5 | 245.16*; 227.04; 203.06; 175.04 | 263.09 | 203.06 | (D/L)-qianhucoumarin G (QC-G) | 80 | 20 | LF | [ |
11 | 19.70 | C14H14O5 | 245.16*; 227.04; 203.06; 175.04 | 263.09 | 203.06 | (D/L)-qianhucoumarin G | 80 | 20 | LF | [ |
12 | 22.31 | C13H10O5 | 189.03 | 264.08 | 189.03 | isopimpinellin | 80 | 25 | LF | [ |
13 | 23.91 | C21H24O9 | 361.13; 245.08 | 438.17 | 245.08 | 3'-(hydroxyl-isovaleryl/2-methylbutyroyl)- 4'-hydroxyl-acetylkhellactone | 80 | 30 | AP | [ |
14 | 24.20 | C21H24O9 | 361.14; 245.08; 187.03 | 438.17 | 245.08 | 3'-(hydroxyl-isovaleryl/2-methylbutyroyl)-4'-hydroxyl-acetylkhellactone | 80 | 30 | AP | [ |
15 | 24.22 | C16H16O6 | 287.10; 245.08 | 305.10 | 245.08 | qianhucoumarin B | 80 | 30 | AP | [ |
16 | 24.86 | C21H24O9 | 361.14; 245.08; 187.03 | 438.17 | 245.08 | 3'-(hydroxyl-isovaleryl/2-methylbutyroyl)-4'-hydroxyl-acetylkhellactone | 80 | 30 | AP | [ |
17 | 25.73 | C12H8O4 | 202.03; 174.06 | 217.04 | 202.03 | bergapten or methoxsalen | 80 | 20 | LF | [ |
18 | 27.17 | C21H22O8 | 343.12; 245.08 | 420.16 | 343.12 | 3'-(hydroxyl-tigloyl/senecioyl/angeloyl)- 4'-acetylkhellactone | 80 | 15 | AP | [ |
19 | 27.57 | C21H22O8 | 343.11; 287.09; 245.08 | 420.16 | 343.11 | 3'-(hydroxyl-tigloyl/angeloyl/senecioyl)- 4'-acetylkhellactone | 80 | 15 | AP | [ |
20 | 29.29 | C16H14O5 | 245.08; 217.08; 175.04 | 287.09 | 245.08 | 3'-acetyloxy-3',4'-dihydroseselin or 4'- acetyloxy-3',4'-dihydroseselin | 80 | 35 | AP | [ |
21 | 29.31 | C18H18O7 | 287.09; 245.08; 227.07 | 369.09 | 245.08 | qianhucoumarin D | 80 | 32 | AP | [ |
22 | 29.85 | C12H8O4 | 202.03; 174.06; 165.72 | 217.04 | 202.03 | bergapten or methoxsalen | 80 | 20 | LF | [ |
23 | 30.70 | C22H26O7 | 329.14; 315.12; 245.08; 227.08 | 420.20 | 343.12 | 3'-(hydroxyl-tigloyl/angeloyl/senecioyl)- 4'-acetylkhellactone | 80 | 15 | AP | [ |
24 | 31.00 | C22H26O7 | 329.14; 315.12; 245.08; 227.07 | 420.20 | 343.12 | 3'-(hydroxyl-tigloyl/angeloyl/senecioyl)- 4'-acetylkhellactone | 80 | 15 | AP | [ |
25 | 31.40 | C22H26O7 | 329.14; 315.12; 245.08; 227.07 | 420.20 | 329.14 | 3'-(isovaleryl/2-methylbutyroyl)-4'-propi- onylkhellactone | 80 | 30 | AP | [ |
26 | 32.15 | C19H20O7 | 287.09; 245.08 | 378.15 | 245.08 | 3'-acetyl-4'-propionylkhellactone | 80 | 30 | AP | [ |
27 | 32.34 | C19H20O7 | 287.09; 245.08 | 378.15 | 245.08 | 3'-acetyl-4'-propionylkhellactone isomer | 80 | 30 | AP | [ |
28 | 33.52 | C19H20O6 | 245.08; 217.07; 205.04; 191.04 | 345.13 | 245.08 | Pd-C-I | 80 | 30 | LP | [ |
29 | 35.18 | C20H22O7 | 287.09; 245.08 | 392.17 | 245.08 | bocconin | 80 | 15 | AP | [ |
30 | 35.61 | C19H20O6 | 345.13; 245.08; 261.05 | 362.15 | 261.05 | 3'-(isovaleryloxy/2-methylbutyroyloxy)- 4'-oxo-3',4'-dihydroseselin | 80 | 30 | AP | [ |
31 | 35.64 | C20H22O7 | 287.09; 245.08 | 392.17 | 245.08 | isobocconin | 80 | 15 | AP | [ |
32 | 36.19 | C19H20O6 | 345.13; 261.06; 245.08 | 362.15 | 261.06 | 3'-(isovaleryloxy/2-methylbutyroyloxy)- 4'-oxo-3',4'-dihydroseselin | 80 | 30 | AP | [ |
33 | 36.27 | C11H6O4 | 175.04; 147.05 | 203.03 | 147.05 | xanthotoxol | 80 | 40 | LF | [ |
34 | 36.45 | C16H14O4 | 203.03; 175.04; 159.03; 147.04; 131.02 | 271.09 | 203.03 | imperatorin | 80 | 18 | LF | [ |
35 | 37.69 | C21H22O7 | 327.12; 287.09; 245.08; 227.07* | 409.12 | 245.08 | (3'S)-pteryxin (D-Pte) | 80 | 20 | AP | [ |
36 | 37.71 | C16H14O5 | 245.08; 217.09; 175.04 | 287.09 | 245.08 | 4'-acetyl-khellactone | 80 | 32 | AP | [ |
37 | 38.04 | C21H22O7 | 327.12; 287.09; 245.08*; 227.07 | 409.12 | 227.07 | (3'S)-praeruptorin A (D-PA) | 80 | 19 | AP | [3,22] |
38 | 38.12 | C24H28O7 | 349.10; 327.12; 251.06; 245.08; 227.07 | 451.17 | 245.08 | 3'-(isovaleryl/2-methylbutyroyl)-4'-(tigloyl/ senecioyl/angeloyl)-khellactone | 80 | 34 | AP | [ |
39 | 38.50 | C17H16O5 | 283.08; 233.04; 218.05; 189.12; 173.03 | 301.10 | 233.04 | cnidilin | 80 | 37 | LF | [ |
40 | 38.94 | C16H14O5 | 245.08; 175.04 | 287.09 | 245.08 | 3'-acety-4'-dehydro-decusinol | 80 | 32 | LP | - |
41 | 39.01 | C23H28O7 | 351.12; 245.08; 227.07 | 434.21 | 245.08 | 3'-(2-isobutyroyl)-4'-(isovaleryl/2-meth- ylbutyroyl)-khellactone | 80 | 30 | AP | [ |
42 | 39.03 | C21H24O7 | 329.13*; 287.09; 245.08 | 406.18 | 245.08 | (3'S)-3'-isovaleryl-4'-acetylkhellactone (D-IAK) | 80 | 30 | AP | [ |
43 | 39.49 | C21H22O7 | 327.12; 287.09; 245.07; 227.07* | 409.12 | 245.08 | (3'R)-pteryxin (L-Pte) | 80 | 20 | AP | [ |
44 | 39.58 | C21H22O7 | 327.12; 287.09; 245.08*; 227.07 | 409.12 | 227.07 | (3'R)-praeruptorin A (L-PA) | 80 | 19 | AP | [ |
45 | 41.42 | C16H14O4 | 203.05; 175.04; 159.03; 147.03; 131.02 | 271.06 | 203.05 | isoimperatorin | 80 | 18 | LF | [ |
46 | 41.60 | C21H24O7 | 329.13*; 287.09; 245.08 | 406.18 | 245.08 | (3'R)-3'-isovaleryl-4'-acetylkhellactone (L-IAK) | 80 | 30 | AP | [ |
47 | 45.84 | C23H26O7 | 315.12; 245.08 | 432.20 | 315.12 | 3'-(2-isobutyroyl)-4'-(tigloyl/senecioyl/angeloyl)-khellactone | 80 | 15 | AP | [ |
48 | 46.05 | C23H26O7 | 327.13; 315.12; 227.09 | 432.20 | 227.09 | 3'-(2-isobutyroyl)-4'-(tigloyl/senecioyl/ angeloyl)-khellactone | 80 | 30 | AP | [ |
49 | 47.41 | C24H26O7 | 327.12; 227.07 | 444.20 | 327.12 | 3'-(tigloyl/senecioyl/angeloyl)-4'-(tigloyl/ senecioyl/angeloyl)-khellactone | 80 | 25 | AP | [3,4 |
50 | 48.16 | C24H26O7 | 327.12; 245.08; 217.08; 189.02 | 444.20 | 327.12 | 3'-(tigloyl/senecioyl/angeloyl)-4'-(tigloyl/ senecioyl/angeloyl)-khellactone | 80 | 26 | AP | [ |
51 | 48.90 | C23H28O7 | 351.13; 245.08; 227.07 | 434.21 | 245.08 | 3'-(2-isobutyroyl)-4'-(isovaleryl/2-meth- ylbutyroyl)-khellactone | 80 | 30 | AP | [ |
52 | 49.65 | C24H26O7 | 349.11; 327.12; 245.08*; 227.06 | 444.20 | 327.12 | (3'S)-praeruptorin B(D-PB) | 80 | 36 | AP | [ |
53 | 50.28 | C24H28O7 | 329.14; 327.12; 227.06; | 451.17 | 245.08 | 3'-(isovaleryl/2-methylbutyroyl)-4'-(tigloyl/ senecioyl/angeloyl)-khellactone | 80 | 34 | AP | [ |
54 | 50.50 | C24H26O7 | 349.11; 327.12; 245.08*; 227.06 | 444.20 | 327.12 | (3'R)-praeruptorin B (L-PB) | 80 | 36 | AP | [ |
55 | 51.61 | C24H28O7 | 349.11; 327.11; 251.06; 245.08; 227.07* | 451.17 | 245.08 | (3'S)-praeruptorin E (D-PE) | 80 | 35 | AP | [ |
56 | 54.31 | C24H28O7 | 349.11; 327.11; 251.06; 245.08; 227.07* | 451.17 | 245.08 | (3'R)-praeruptorin E (L-PE) | 80 | 35 | AP | [ |
57 | 55.08 | C24H30O7 | 329.13; 245.08*; 227.06 | 448.23 | 227.06 | (3'S)-3',4'-diisovalerylkhellactone (D-DIK) | 80 | 55 | AP | [ |
58 | 56.49 | C24H30O7 | 329.13; 245.08*; 227.06 | 448.23 | 227.06 | (3'R)-3',4'-diisovalerylkhellactone (L-DIK) | 80 | 55 | AP | [ |
59 | 63.18 | C24H30O7 | 329.13; 245.08; 227.06 | 448.23 | 227.06 | 3',4'-diisovalerylkhellactone isomer | 80 | 55 | AP | |
60 | 64.75 | C24H26O7 | 327.12; 245.08; 217.08; 189.02 | 444.20 | 327.12 | 3'-(tigloyl/senecioyl/angeloyl)-4'-(tigloyl/ senecioyl/angeloyl)-khellactone | 80 | 36 | AP | [ |
Fig. 2 Extracted ion current chromatograms of Ligustici Radix using (a) achiral LC-MS/MS and (b) achiral-chiral LC-MS/MS Peaks 1-60 are the same as that in Table 1.
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[10] | YUAN Liming. Progress of chiral countercurrent chromatography [J]. Chinese Journal of Chromatography, 2016, 34(1): 44-49. |
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