[an error occurred while processing this directive]
Chinese Journal of Chromatography
   Home   |   About Journal   |   Editorial Board   |   Impact Factor   |   Indexed In   |   Aims and Scope   |   Download   |   Subscriptions   |   FAQ   |   Contact Us   |   Chinese
Chinese Journal of Chromatography  2015, Vol. 33 Issue (8): 838-842    DOI: 10.3724/SP.J.1123.2015.04016
Current Issue | Next Issue | Archive | Adv Search  |   
Determination of five β2-agonist residues in pork by ultra-high performance liquid chromatography-tandem mass spectrometry with molecularly imprinted solid phase extraction
ZHANG Xueliang1, LUO Yunjing1, JIANG Jie2, LU Yong2, FENG Nan2
1. College of Life Sciences & Bio-Engineering, Beijing University of Technology, Beijing 100124, China;
2. Beijing Municipal Food Safety Monitoring and Risk Assessment Center, Beijing 100041, China
 Download: [PDF 1376KB]   [Full Text(HTML)] ()   Export: BibTeX | EndNote (RIS)      Supporting Info
Abstract 

An ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method with molecularly imprinted solid phase extraction for the determination of five β2-gonists residues in pork has been developed. After the sample preparation, the ammonium acetate/acetic acid buffer was added, followed by the internal standard and β-glucuronidase/arylsulfatase enzyme. The solution was incubated at 55 ℃ for 2 h. After adjusting the pH of the solution, it was purified by a molecularly imprinted solid phase extraction column, then analyzed on a BEH C18 column with methanol-0.1% (v/v) formic acid aqueous solution as the mobile phases in gradient elution mode. The MS/MS analysis was in positive ion mode and multiple reaction monitoring mode. The analytes were quantified by the internal standard method. The limits of detection (LODs, S/N=3) and the limits of quantification (LOQs, S/N=10) were 0.005-0.009 μg/kg and 0.015-0.025 μg/kg, respectively. In the range of 0-10 μg/kg, the correlation coefficients of linear calibration curves were not less than 0.9933. At the spiked levels of 0.25, 1.0 and 5.0 μg/kg, the recoveries were 80.4%-92.9% with the relative standard deviations of 1.3%-6.3%. The method is of high sensitivity, good reproducibility, high recovery, and is useful for the simultaneous determination of multiple β2-agonists residues.

Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
ZHANG Xueliang
LUO Yunjing
JIANG Jie
LU Yong
FENG Nan
Key wordsultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)   molecularly imprinted solid phase extraction   β2-gonists   residues   pork     
Received: 2015-04-13;
Cite this article:   
ZHANG Xueliang,LUO Yunjing,JIANG Jie et al. Determination of five β2-agonist residues in pork by ultra-high performance liquid chromatography-tandem mass spectrometry with molecularly imprinted solid phase extraction[J]. Chinese Journal of Chromatography, 2015, 33(8): 838-842.
 
No references of article
[1] ZHU Wanyan, ZHANG Xin, YANG Juan, XU Wenyuan, XU Meiling. Simultaneous determination of multi-classes of veterinary drug residues in pork by ultra performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry[J]. Chinese Journal of Chromatography, 2015, 33(9): 1002-1008.
[2] WU Chunying, GU Feng, BAI Lu, LU Wenlong. Simultaneous determination of 22 typical pharmaceuticals and personal care products in environmental water using ultra performance liquid chromatography- triple quadrupole mass spectrometry[J]. Chinese Journal of Chromatography, 2015, 33(8): 843-848.
[3] LIU Xiaoqin, TONG Ling, MENG Wenting, SUN Guoxiang. Determination of 99 pesticide residues in Paeoniae Radix Alba by gas chromatography-triple quadrupole tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2015, 33(8): 869-877.
[4] CHENG Guodong, WU Xiaohui, JIN Zhu, ZHANG Yu, HAO Dan, TONG Mianhuan, GAO Jianjun. Simultaneous determination of three sulfonamide residues in modified milk by ultra performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2015, 33(8): 892-896.
[5] QIU Panzi, GUO Xinyan, WANG Na, KONG Xiangji, HE Hua. Simultaneous determination of ten antibiotics in pharmaceutical wastewater using ultra-high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2015, 33(7): 722-729.
[6] LI Yong, LIN Qian, PANG Tao, SHI Junli. Determination of 12 flavonoids in tobacco leaves using ultra-high performance liquid chromatography- tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2015, 33(7): 746-752.
[7] GOU Xinlei, ZHAO Xinying, CHI Haitao, GAO Xia, ZHOU Mingqiang, LIU Weili. Simultaneous determination of ten benzotriazole ultraviolet stabilizers in food contact plastic materials by solid phase extraction and ultra performance liquid chromatography with tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2015, 33(6): 571-576.
[8] WEI Hairong, YI Xibin, TAN Yue, ZONG Xiaojuan, WANG Jiawei, XU Li, LIU Qingzhong. Determination of anthocyanins in the peel of sweet cherry by ultra performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2015, 33(6): 577-582.
[9] YU Lu, SONG Wei, LÜ Yaning, ZHAO Muyu, ZHOU Fangfang, HU Yanyun, ZHENG Ping. Rapid determination of 204 pesticide residues in tea by ultra performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry[J]. Chinese Journal of Chromatography, 2015, 33(6): 597-612.
[10] CAO Xinyue, PANG Guofang, JIN Linghe, KANG Jian, HU Xueyan, CHANG Qiaoying, WANG Minglin, FAN Chunlin. Comparison of the performances of gas chromatography- quadrupole time of flight mass spectrometry and gas chromatography-tandem mass spectrometry in rapid screening and confirmation of 208 pesticide residues in fruits and vegetables[J]. Chinese Journal of Chromatography, 2015, 33(4): 389-396.
[11] GONG Liping, SHI Feng, JIANG Shuyin, ZHAO Yanxia, GUO Changchuan. Determination of nine pesticide residues in vegetables by matrix solid-phase dispersion-ultra performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2015, 33(4): 408-412.
[12] WU Yan, JIANG Bing, XU Yigang, ZHAO Wei, MENG Xiangrui, ZHOU Yuan, YU Jiahui, ZU Yuangang. Determination of 16 pesticide residues in fruits and vegetables by QuEChERS-liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2015, 33(3): 228-234.
[13] XU Juan, WANG Lan, HUANG Huajun, CHEN Jie, CHEN Wenrui, XIANG Dapeng. Low temperature freezing followed by dispersive solid phase extraction for the determination of 104 pesticide residues in vegetable oils using ultra-performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2015, 33(3): 242-249.
[14] LIN Tao, SHAO Jinliang, LIU Xingyong, YANG Dongshun, CHEN Xinglian, LI Yangang, FAN Jianlin, LIU Hongcheng. Determination of 41 pesticide residues in vegetables by QuEChERS-ultra performance liquid chromatography- tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2015, 33(3): 235-241.
[15] CAO Yafei, KANG Jian, CHANG Qiaoying, HU Xueyan, WANG Zhibin, FAN Chunlin, WANG Minglin. Multi-residue determination of veterinary drugs in cheese by QuEChERS and liquid chromatography- tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2015, 33(2): 132-139.
?

Copyright © 2010 Editorial Office of Chinese Journal of Chromatography, Dalian Institute of Chemical Physics, CAS   辽ICP备10003855号
457 Zhongshan Road,116023, Dalian,P.R.China
Tel: +86-411-84379021, Fax: +86-411-84379600    E-mail: sepu@dicp.ac.cn

Supported by:Beijing Magtech

美女图片

美女

美女图片