Determination of imidazoles and their metabolites in pork by liquid chromatography-tandem mass spectrometry

doi:10.3724/SP.J.1123.2017.06029

Abstract

Abstract:

A method for the determination of 29 imidazoles and their metabolites in pork by liquid chromatography-tandem mass spectrometry has been developed. The samples were extracted with ethyl acetate, and then defatted with n-hexane after concentration of the extracts. The analytes were separated on a C₁₈ reversed-phase column with 0.3% (v/v) formic acid aqueous solution and acetonitrile as mobile phases, and finally analyzed using electrospray ionization in positive ion mode (ESI⁺) with multiple reaction monitoring (MRM). The linear ranges of the 29 compounds were from 0.05 to 20.0 μg/L with the correlation coefficients (r²) more than 0.99. The average recoveries and relative standard deviations were 65.4%-103% and 1.3%-6.8% respectively in the spiked ranges of 1.0-5.0 μg/kg. The limits of detection and the limits of quantification were 0.02-0.3 μg/kg and 0.1-1 μg/kg respectively for the 29 imidazoles. The method is simple, rapid and sensitive. It is suitable for the detection of imidazoles and their metabolites in pork.

Key words: imidazoles, liquid chromatography-tandem mass spectrometry (LC-MS/MS), pork, residue

CLC Number:

O658

YANG Wanghuo, WU Shaoming, CAI Xiaoming, FENG Lifeng, CHEN Yankai, LI Yanping, LIANG Min. Determination of imidazoles and their metabolites in pork by liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2017, 35(10): 1062-1067.

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