色谱

色谱

• 特别策划 • 上一篇    

动态磁涂覆混合固定相开管毛细管电色谱中电渗流的特征

覃飒飒1, 周超然2, 朱亚仙1, 任志宇2, 张凌怡1, 付宏刚2*, 张维冰1*   

  1. 1. 华东理工大学, 上海市功能性材料化学重点实验室, 上海 200237; 2. 黑龙江大学, 功能无机材料化学省部共建教育部重点实验室, 黑龙江 哈尔滨 150080
  • 收稿日期:2011-07-18 修回日期:2011-08-01 出版日期:2011-09-28 发布日期:2011-10-28
  • 通讯作者: 张维冰,博士,教授,主要从事色谱理论与实践研究. 付宏刚,博士,教授,主要从事半导体光电材料和纳米晶态碳基材料等方面的研究.
  • 基金资助:

    国家重大基础研究项目(批准号: 2007CB914100, 2011CB910404)和国家自然科学基金项目(批准号: 21075039, 20971040).

Characteristics of electroosmotic flow in open-tubular capillary electrochromatography with magnetic nanoparticle coating as mixed-mode stationary phase

QIN Sasa1, ZHOU Chaoran2, ZHU Yaxian1, REN Zhiyu2, ZHANG Lingyi1, FU Honggang2*, ZHANG Weibing1*   

  1. 1. Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, China; 2. Key Laboratory of Functional Inorganic Material Chemistry (Ministry of Education), Heilongjiang University, Harbin 150080, China
  • Received:2011-07-18 Revised:2011-08-01 Online:2011-09-28 Published:2011-10-28

PDF

505

被引次数

10

摘要： 将表面分别被改性成C18和氨基的磁性纳米颗粒按照不同比例混合,制备成具有不同分离选择性的混合固定相,进一步采用动态磁涂覆的方法制备开管毛细管电色谱柱。通过考察这种色谱柱中不同种类固定相表面物理化学性质对电渗流的综合影响,从理论上说明了其电渗流的特征。分别在不同固定相配比及不同涂覆长度条件下进行实验,理论与实验结果相符,证实通过调节固定相配比或磁铁对数可以便捷地调节电渗流的大小。

关键词: 电渗流, 毛细管电色谱, 纳米固定相

Abstract: Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, China; 2. Key Laboratory of Functional Inorganic Material Chemistry (Ministry of Education), Heilongjiang University, Harbin 150080, China) Abstract: A novel open-tubular capillary electrochromatography (OT-CEC) column with magnetic nanoparticle coating as mixed-mode stationary phase was prepared. The mixed-mode stationary phases were obtained by mixing C18 and amino modified magnetic nanoparticles with different ratios. The mixed modified magnetic nanoparticles as stationary phase were introduced into the capillary by using external magnetic force. The magnetic nanoparticle coating can be easily regenerated by removing the external magnetic field, and applied to other separation modes. The characteristics of electroosmotic flow (EOF) were theoretically investigated through the effect of physicochemical properties of different stationary phases on EOF. The experiment was conducted under different ratios of mixed-mode stationary phases and coating lengths, and it was verified that the theoretical conclusions accorded with the experimental results. It was shown that the EOF can be easily adjusted by changing the ratio of stationary phases or the number of permanent magnets.

Key words: electroosmotic flow, nano stationary phase, capillary electrochromatography （CEC）