色谱

色谱 ›› 2016, Vol. 34 ›› Issue (2): 152-157.DOI: 10.3724/SP.J.1123.2015.08029

• 研究论文 • 上一篇    下一篇

(4-环戊二烯基苯甲酸-铁-甲苯)六氟磷酸盐键合硅胶固定相的制备及色谱性能

曹爱娟1, 李晓乐2, 乔丽君1, 周晓华1, 于阿娟1, 张书胜1, 吴养洁1   

  1. 1. 郑州大学化学与分子工程学院, 河南 郑州 450001;
    2. 济源职业技术学院冶金化工系, 河南 济源 459000
  • 收稿日期:2015-08-25 出版日期:2016-02-08 发布日期:2012-09-11
  • 通讯作者: 于阿娟
  • 基金资助:

    国家自然科学基金项目(21205107,21275133,21475119).

Preparation and chromatographic performance of a silica-bonded (4-cyclopentadienyl benzoic acid-iron-toluene) hexafluorophosphoric acid stationary phase

CAO Aijuan1, LI Xiaole2, QIAO Lijun1, ZHOU Xiaohua1, YU Ajuan1, ZHANG Shusheng1, WU Yangjie1   

  1. 1. College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, China;
    2. Metallurgical and Chemical Department, Jiyuan Vocational and Technical College, Jiyuan 459000, China
  • Received:2015-08-25 Online:2016-02-08 Published:2012-09-11
  • Supported by:

    National Natural Science Foundation of China (Grant Nos. 21205107, 21275133, 21475119).

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摘要：

基于二茂铁独特的分子结构及其作为新型液相色谱分离基质的潜质,制备了新型(4-环戊二烯基苯甲酸-铁-甲苯)六氟磷酸盐键合硅胶固定相,利用红外光谱、元素分析、热重分析等手段对其进行了表征。分别选取多环芳烃、萘胺位置异构体、硝基苯胺位置异构体、硝基咪唑类化合物和有机磷化合物溶质作为探针,对其色谱性能进行了评价。结果表明,该色谱固定相能提供 π-电子转移、 π-π 作用、偶极-偶极作用及与底物的静电相互作用等多种作用力,并探讨了可能的分离机理。

关键词: (4-环戊二烯基苯甲酸-铁-甲苯)六氟磷酸盐, 分离机理, 高效液相色谱, 固定相

Abstract:

Based on the unique molecular structure of ferrocene and its potential as a new liquid chromatography separation medium, a new silica-bonded (4-cyclopentadienyl benzoic acid-iron-toluene) hexafluorophosphoric acid stationary phase was prepared. The structure of this new material was characterized by infrared spectroscopy, elemental analysis, thermogravimetric analysis et al. The chromatographic performance and retention mechanism of this new stationary phase were evaluated using different solute probes, including polycyclic aromatic hydrocarbons (PAHs), positional isomers of naphthylamine, positional isomers of nitro-aniline, nitroimidazoles, organic phosphorus et al. It could provide various action sites for different solutes in normal-phase chromatography such as π electron transfer, π-π electron interactions, dipole-dipole interactions, and electrostatic interactions with the substrates. And the possible separation mechanisms are discussed.

Key words: (4-cyclopentadienyl benzoic acid-iron-toluene) hexafluorophosphoric acid, high performance liquid chromatography (HPLC), separation mechanism, stationary phase

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