色谱

色谱

• •    

仪器分析综合实验：磁性多功能材料的制备及在磷酸化肽富集和分析中的应用

李松芮1,2,*, 郑海娇1,2, 赵亚明1,2, 卫新壮1, 贾琼1,2,*   

  1. 1.吉林大学化学学院, 吉林 长春 130012;
    2.吉林大学国家级化学实验教学示范中心, 吉林 长春 130012
  • 收稿日期:2026-03-19
  • 通讯作者: *Tel: 0431-85168436, E-mail: lisongrui@jlu.edu.cn（李松芮）; Tel: 0431-85168436，E-mail: jiaqiong@jlu.edu.cn（贾琼）.
  • 基金资助:
    国家自然科学基金青年项目（22506052）; 吉林大学本科教育教学改革研究项目（教学成果专项）（25CG097）.

Comprehensive instrumental analysis experiment: preparation and application of multifunctionalized magnetic material for the enrichment and analysis of phosphopeptides

LI Songrui1,2,*, ZHENG Haijiao1,2, ZHAO Yaming1,2, WEI Xinzhuang1, JIA Qiong1,2,*   

  1. 1. College of Chemistry, Jilin University, Changchun 130012, China;
    2. National Demonstration Center for Experimental Chemistry Education, Jilin University, Changchun 130012, China
  • Received:2026-03-19
  • Supported by:
    Youth Fund of the National Natural Science Foundation of China（No. 22506052）; Undergraduate Education and Teaching Reform Research Project of Jilin University（Special Program for Teaching Achievements）（No. 25CG097）.

PDF

6

摘要： 针对当前仪器分析实验教学多局限于单一验证性实验、内容相对独立、缺乏与前沿科研热点相融合的现状,我们设计了一个包含材料合成与表征、磷酸化肽吸附性能及检测方法探究的本科生仪器分析综合实验。首先,采用多巴胺辅助共沉淀策略制备磁性多功能材料;利用红外光谱仪、X射线衍射仪和热重分析仪等仪器设备对材料的理化性能进行表征;随后,以磁性多功能材料作为磁性固相萃取（MSPE）平台的载体,结合基质辅助激光解吸电离飞行时间质谱（MALDI‑TOF MS）,应用于脱脂牛奶样品中低丰度磷酸化肽的高效富集与检测。本实验设计将前沿科研热点有效融入本科教学,通过多仪器结合使用与多学科交叉,显著提升了学生的综合实验技能与解决复杂实际问题的能力。

关键词: 综合性实验, 仪器分析, 质谱, 磁性固相萃取, 磷酸化肽

Abstract: At present, the experimental teaching of instrumental analysis is plagued by several issues, such as being largely limited to single confirmatory experiments, relatively independent content, and a lack of integration with scientific research hotspots. Aiming at the above problems, a comprehensive instrumental analysis experiment for undergraduate students was designed. This experiment covers the synthesis and characterization of materials, as well as the investigation of adsorption and detection methods of phosphopeptides. Firstly, a multifunctionalized magnetic material was prepared. The physicochemical properties of the magnetic material were characterized by means of Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and other instrumental techniques. Subsequently, the magnetic material was used as the carrier of the magnetic solid phase extraction (MSPE) platform to enrich phosphopeptides. Combined with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), the detection of low-abundance phosphopeptides in defatted milk samples was achieved. This experiment effectively integrates scientific research hotspots into basic undergraduate teaching. Through the combined application of multiple instruments and interdisciplinary integration, students’ comprehensive experimental skills and ability to solve complex practical problems have been significantly enhanced.

Key words: comprehensive experiment, instrumental analysis, mass spectrometry, magnetic solid phase extraction (MSPE), phosphopepties

中图分类号: