色谱

色谱

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本科生研究型实验：双金属普鲁士蓝纳米酶的制备及其比色检测双酚A

张羽, 周新月, 马慕瑶, 任露, 廖苑君, 许旭*, 张蕾   

  1. 辽宁大学化学院, 辽宁 沈阳 110036
  • 收稿日期:2026-06-23
  • 通讯作者: *Tel:024-62202380,E-mail：xuxu@lnu.edu.cn.
  • 作者简介:张羽. Tel:024-62202380, E-mail：yu_zhang_chem@126.com. 本科,学生.
  • 基金资助:
    辽宁大学本科教学改革项目(JG2024BYLW03, JG2025CSYJ15); 国家自然科学基金(32102086); 辽宁省自然科学基金面上项目(2026-MS-117).

Undergraduate research experiment: preparation of bimetallic Prussian blue nanozyme and its application in colorimetric detection of bisphenol A

ZHANG Yu, ZHOU Xinyue, MA Muyao, REN Lu, LIAO Yuanjun, XU Xu*, ZHANG Lei   

  1. College of Chemistry, Liaoning University, Shenyang 110036, China
  • Received:2026-06-23
  • Supported by:
    Liaoning University Undergraduate Teaching Reform Project (Nos. JG2024BYLW03, JG2025CSYJ15); National Natural Science Foundation of China (No. 32102086); Liaoning Provincial Natural Science Foundation General Project (No. 2026-MS-117).

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摘要： 在教育部大力推进新工科建设和学科交叉融合的教育改革背景下,如何将前沿科研成果有效转化为优质教学资源,是化学实验教学改革的核心问题。本研究依托面向化学类专业大三本科生独立开设的《研究型实验》课程（48学时）,设计并实施了一项核心探究性实验模块,实验合成双金属普鲁士蓝类似物CuCo-PBA纳米酶材料,系统研究其类氧化酶催化性能。基于该纳米酶在无H2O2条件下可直接催化显色底物3,3′,5,5′-四甲基联苯胺(TMB)氧化,生成蓝色的氧化态TMB,结合双酚A(BPA)的还原特性使氧化态TMB褪色的机制,构建了一种简单、高效的比色传感检测体系。实验内容涵盖材料合成、结构表征、酶活性验证、动力学研究、方法学验证及实际样品分析等完整科研流程。通过本课程的系统训练,学生深入理解了纳米材料结构与性能的关系,显著提升了实验设计、数据分析及学术表达能力。课程将前沿科研成果与实验教学有机融合,使科学精神培养与价值引领在文献综述与方案设计、核心实验操作、数据处理及论文撰写、汇报答辩等各环节中同步发生,有效激发了学生的科研兴趣与社会责任感,为培养高素质复合型人才奠定了坚实基础。

关键词: 研究型实验, 纳米酶, 比色检测, 教学改革

Abstract: Against the backdrop of the Ministry of Education's vigorous promotion of the New Engineering initiative and interdisciplinary integration, effectively translating cutting-edge scientific research into high-quality educational resources has emerged as a core issue in chemistry experiment teaching reform. Relying on the independently offered Research-oriented Experiments course (48 credit hours) for junior undergraduate students majoring in chemistry, this study designed and implemented a core inquiry-based experimental module. In this module, a bimetallic Prussian blue analogue (CuCo-PBA) nanozyme was synthesized, and its oxidase-like catalytic performance was systematically investigated. Based on the characteristic that this nanozyme can directly catalyze the oxidation of the chromogenic substrate TMB to generate a blue product without H2O2, a simple and highly efficient colorimetric sensing system was constructed by utilizing the color-lightening effect caused by the reduction of bisphenol A (BPA). The experimental content covers the complete scientific research process, including material synthesis, structural characterization, enzyme activity verification, kinetic studies, methodology validation, and actual sample analysis. Through systematic training in this course, students gained an in-depth understanding of the structure-property relationship of nanomaterials, and their abilities in experimental design, data analysis, and academic expression were significantly enhanced. The course organically integrates frontier scientific achievements into experimental teaching, ensuring that the cultivation of scientific spirit and value guidance occurs synchronously across all stages, including literature review, experimental inquiry, and application expansion. This approach effectively stimulates students' interest in scientific research and their sense of social responsibility, laying a solid foundation for cultivating high-quality, versatile talents.

Key words: research-oriented experiment, nanozyme, colorimetric detection, teaching reform

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