Abstract: A capillary zone electrophoresis method (CZE) for the simultaneous determination of loureirin A and loureirin B was developed based on the optimized conditions of the pH, composition and concentration of the running buffer solution. Loureirin A and loureirin B were separated and determined effectively within 15 min in a running buffer solution of 20 mmol/L Na2B4O7 (pH 9.98 adjusted with NaOH solution) containing 10.0%(v/v) acetonitrile, 5.0%(v/v) ethylene glycol and 1.0%(v/v) butanol, with the applied voltage of 20 kV, capillary temperature of 25 ℃, detection wavelength of 211 nm, and injection of 5 s at 3447 Pa. The linear ranges for the determination of loureirin A and loureirin B were 1.00~100 mg/L and 0.50~100 mg/L, respectively. The determination of loureirin A and loureirin B in dragon’s blood from natural and artificial inoculation was performed by the proposed method. The relative standard deviations for the determination of the two constituents in samples were from 0.6% to 3.8%, and the recoveries ranged between 95.1% and 105.8%. The method is simple, rapid and possesses higher reproducibility and efficiency. It can be used for the determination of loureirin A and loureirin B in dragon’s blood.

Key words: dragon’s blood, loureirin A, loureirin B, capillary zone electrophoresis (CZE)