Abstract: An LC-MS/MS method was developed for the simultaneously determination of seven strobilurin fungicide residues in Chinese herbs. The strobilurin fungicides include Z-metominostrobin, kresoxim-methyl, dimoxystrobin, picoxystrobin, pyraclostrobin, azoxystrobin and trifloxystrobin. The sample was extracted with ethyl acetate and cleaned-up by an amino SPE column. The seven strobilurin fungicide residues were separated on a C18 column with gradient elution of 1.0‰ formic acid and methanol as mobile phases, and detected by ESI-MS in positive ion and selective reaction monitoring （SRM） mode. External standard method was used to the quantification with good linear relationships （r≥0.996）. The LOQs were 2 μg/kg for dimoxystrobin, picoxystrobin and trifloxystrobin, 4 μg/kg for pyraclostrobin and azoxystrobin, 10 μg/kg for Z-metominostrobin and kresoxim-methyl. The recoveries were from 60.4% to 110% with the RSDs between 1.2% and 17%. The developed method is suitable for the determination and confirmation of the seven strobilurin fungicide residues in the three of Eight Zhes （Ophiopogon japonicus （Thunb.）, Scrophularia ningpoensis Hemsl. and Corydalis yanhusuo W T Wang）.

Key words: Chinese herbs, liquid chromatography-tandem mass spectrometry (LC-MS/MS）, strobilurin fungicides, solid phase extraction（SPE）