Chinese Journal of Chromatography ›› 2013, Vol. 31 ›› Issue (6): 514-517.DOI: 10.3724/SP.J.1123.2013.01008

• Quantitative Proteomics • Previous Articles     Next Articles

Identification of methylation at lysine 27 and 36 sites in histone H3

WANG Guojuan1,2, ZHANG Kai1,2, HE Xiwen2, ZHANG Yukui1,2,3   

  1. 1. State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, China;
    2. College of Chemistry, Nankai University, Tianjin 300071, China;
    3. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
  • Received:2013-01-05 Revised:2013-03-11 Online:2013-06-28 Published:2013-06-06
  • Contact: 10.3724/SP.J.1123.2013.01008

Abstract:

we combined high performance liquid chromatography and tandem mass spectrometry (HPLC-MS/MS) with bioinformatics tools to analyze the isobaric modified peptides which were methylated and dimethylated at either lysine (K) 27 or/and K36 from histone H3. They were identified and dissected through alignment of every fragment ion, and the two modified sites were further analyzed according to their relative intensities of MS/MS spectra.

Key words: histone, liquid chromatography (LC), mass spectrometry (MS), post-translational modifications (PTMs), quantitative proteomics

CLC Number: