Purification and characterization of the biosurfactant rhamnolipid

doi:10.3724/SP.J.1123.2013.10026

Abstract

Abstract:

Biosurfactant rhamnolipid is a metabolic intermediate produced by microorganisms under a certain condition. There are the polar hydrophilic group and the non-polar hydrophobic group in rhamnolipid molecule which always exhibits high surface or interfacial activity. A reliable separation and purification method as well as component identification technique is essential for success of production process. The rhamnolipid was produced by aerobic fermentation using Pseudomonas aeruginosa CCTCC AB93066 in this study. It was separated from the culture by acid precipitation and purified by column chromatography until two groups of monorhamnolipid and dirhamnolipid were obtained. High performance liquid chromatography with mass spectrometry (HPLC-MS) examination showed that either the monorhamnolipid or the dirhamnolipid contained three major species. They were RhaC₁₀C₁₀, RhaC₁₀C₁₂-H₂, RhaC₁₀C₁₂ for monorhamnolipid and Rha₂C₁₀C₁₀, Rha₂C₁₀C₁₂-H₂, Rha₂C₁₀C₁₂ for dirhamnolipid. The results of the study suggested that Pseudomonas aeruginosa CCTCC AB93066 is a good strain for rhamnolipid production. Acid precipitation-column chromatography technique is good for purification of rhamnolipid. Meanwhile, HPLC-MS is a reliable method for identifying components of rhamnolipid with high sensitivity and accuracy.

Key words: Pseudomonas aeruginosa, acid precipitation, biosurfactant, column chromatography, high performance liquid chromatography-mass spectrometry (HPLC-MS), rhamnolipid

CLC Number:

O658

LIU Yang, ZHONG Hua, LIU Zhifeng, JIANG Yongbing, TAN Fei, ZENG Guangming, LAI Mingyong, HE Yibin. Purification and characterization of the biosurfactant rhamnolipid[J]. Chinese Journal of Chromatography, 2014, 32(3): 248-255.

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