Simultaneous determination of four Sudan dyes in blood by solid-phase extraction combined with ultra-fast liquid chromatography-tandem mass spectrometry

doi:10.3724/SP.J.1123.2013.11005

Abstract

Abstract:

A method for the simultaneous determination of Sudan Ⅰ, Ⅱ, Ⅲ, and Ⅳ in blood samples by solid-phase extraction (SPE) combined with ultra-fast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) has been established. The samples were extracted with acetonitrile by vortex and vibrate technique, and then the supernatant was diluted with equal volume of water and cleaned up by a C18 SPE column. The separation was performed on an Agilent Eclipse Plus C18 column (100 mm×2.1 mm, 1.8 μm) by gradient elution with acetonitrile containing 0.1% (v/v) formic acid and 0.1% (v/v) formic acid aqueous solution as the mobile phases. The electrospray ionization (ESI) source in the positive mode and multiple reaction monitoring (MRM) mode were used for the quantitative analysis. In addition, the phenomenon of E-Z optical isomer occurred by the azo group from Sudan Ⅲ and Ⅳ was found, and the influencing factors were discussed. The results showed that the calibration curves were in good linearity for the four Sudan dyes ranged from 0.1 to 20.0 μg/L with the correlation coefficients of more than 0.999. The average recoveries were from 93.0% to 108.2% with the relative standard deviations (RSDs) from 4.8% to 9.5%. The limits of detection (LODs) and the limits of quantification (LOQs) were 0.06 μg/L and 0.2 μg/L for the four analytes, respectively. The developed method is simple, rapid, and highly sensitive. It can be used for the determination of trace Sudan dyes in blood samples.

Key words: blood, solid-phase extraction (SPE), Sudan dye, ultra-fast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS)

CLC Number:

O658

ZHU Hao, HUANG Kunyu, FU Jianfei, HU Yue, HUANG Xianni, CHEN Xiaohong, ZOU Baobo, JIN Micong. Simultaneous determination of four Sudan dyes in blood by solid-phase extraction combined with ultra-fast liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2014, 32(3): 224-229.

References

[1] Rebane R, Leito I, Yurchenko S, et al. J. Chromatogr A, 2010, 1217 (17): 2747
[2] Stiborová M, Martínek V, Rýdlová H, et al. Cancer Res, 2002, 62 (20): 5678
[3] European Food Safety Authority. EFSA J, 2005, 263: 1
[4] Ahlstrom L H, Eskilsson C S, Bjorklund E. Trends Anal Chem, 2005, 24 (1): 49
[5] Valencia M C, Uroz F, Tafersiti Y, et al. Quim Anal, 2000, 19 (3): 129
[6] Pang Y L, Wang H Y. Chinese Journal of Analysis Laboratory (庞艳玲, 王怀友. 分析试验室), 2008, 26 (1): 60
[7] Mo Z R, Zhang Y F, Zhao F Q, et al. Food Chem, 2010, 121 (1): 233
[8] Qi Y H, Shan W C, Liu Y Z, et al. J Agric Food Chem, 2012, 60 (9): 2116
[9] Mölder K, Künnapas A, Herodes K, et al. J Chromatogr A, 2007, 1160 (1/2): 227
[10] Zacharis C K, Kika F S, Tzanavaras P D, et al. Talanta, 2011, 84 (2): 480
[11] Noguerol-Cal R, López-Vilario J M, Fernández-Martínez G, et al. J Chromatogr A, 2008, 1179 (2): 152
[12] Liu J, Gong Z B. Chinese Journal of Chromatography (刘珺, 弓振斌. 色谱), 2012, 30 (6): 624
[13] He L M, Su Y J, Fang B H, et al. Anal Chim Acta, 2007, 594 (1): 139
[14] He R, Liao L C, Yan Y Y, et al. Journal of Instrumental Analysis (何荣, 廖林川, 颜有仪, 等. 分析测试学报), 2008, 27 (7): 751
[15] Erdemir U S, Izgi B, Gucer S. Anal Methods, 2013, 5 (7): 1790
[16] Huang X L, Wu H Q, Huang F, et al. Journal of Instrumental Analysis (黄晓兰, 吴惠勤, 黄芳, 等. 分析测试学报), 2005, 24 (3): 1
[17] Schummer C, Sassel J, Bonenberger P, et al. J Agric Food Chem, 2013, 61 (9): 2284
[18] Murty M R V S, Sridhara C N, Prabhakar S, et al. Food Chem, 2009, 115 (4): 1556
[19] Gao H, Yang M L, Wang M L, et al. J AOAC Int, 2013, 96 (1): 110
[20] Li C, Wu Y L, Shen J Z. Food Addit Contam A, 2010, 27 (9): 1215
[21] Wang P, Guo S F, Jing T, et al. Chinese Journal of Chromatography (王鹏, 郭少飞, 荆涛, 等. 色谱), 2008, 26 (3): 353
[22] Mejia E, Ding Y, Mora M F, et al. Food Chem, 2007, 102 (4): 1027
[23] Polson C, Sarkar P, Incledon B, et al. J Chromatogr B, 2003, 785 (2): 263