Analysis of alkaline CuO degradation products of acid detergent fiber from tobacco leaves by using liquid chromatography

doi:10.3724/SP.J.1123.2015.01043

Abstract

Abstract:

The acid detergent fiber (ADF) from tobacco leaves was obtained by treating the sample with petroleum ether-ethanol (6 : 4, v/v), 30 g/L sodium dodecylsulfate and 0.5 mol/L sulphuric acid containing 20 g/L hexadecyl trimethyl ammonium bromide successively. The ADF was degraded by the alkaline CuO oxidation procedure. In this work, six samples of ADF degradation products from tobacco leaves were prepared. The samples were analyzed by using gradient liquid chromatography (LC) where an Ultimate XB C18 column was used as stationary phase, with a mixture of methanol and water as mobile phase, at a column temperature of 35 ℃ and a flow rate of 0.8 mL/min. Dual wavelengths of 280 nm and 320 nm were chosen for the detection. It was found that there were four characteristic peaks for the ADF degradation products. By taking these peaks as research object, the optimum time for the degradation was found to be 5 h and the sample solution could be kept stable within 7 days. The established method may provide a new approach for the studies of the differences between lignin composition in different tobacco leaves and the relationship between lignin content and the smoking quality of tobacco leaves.

Key words: acid detergent fiber (ADF), alkaline CuO degradation, lignin, liquid chromatography (LC), tobacco leaves

CLC Number:

O658

HAO Weiqiang, WANG Leijun, WU Shun, YUE Bangyi, CHEN Qiang, ZHANG Peipei. Analysis of alkaline CuO degradation products of acid detergent fiber from tobacco leaves by using liquid chromatography[J]. Chinese Journal of Chromatography, 2015, 33(7): 777-782.

References

[1] Liu C K, Cai J, Yang J, et al. Guangdong Agricultural Sciences (刘春奎, 蔡佳, 杨靖, 等. 广东农业科学), 2012(12): 33

[2] Zhang Q, Dong G F, Huang W, et al. Tobacco Science & Technology (张强, 董高峰, 黄韡, 等. 烟草科技), 2014(3): 76

[3] Cao S Y, Hu W R, Fan L. Polymer Bulletin (曹双瑜, 胡文冉, 范玲. 高分子通报), 2012(3): 8

[4] Vanholme R, Morreel K, Ralph J, et al. Curr Opin Plant Biol, 2008, 11(3): 278

[5] Li H T, Yao K, Jia D Y, et al. Leather Science and Engineering (李海涛, 姚开, 贾冬英, 等. 皮革科学与工程), 2011, 21(2): 31

[6] Goff B M, Murphy P T, Moore K J. J Sci Food Agric, 2012, 92(4): 751

[7] Yang B, Yin Y, Zhang H B, et al. Acta Tabacaria Sinica (杨斌, 殷引, 张浩博, 等. 中国烟草学报), 2012, 18(3): 10

[8] Guo X Y, Dai Y H, Guo Z M, et al. Tobacco Science & Technology (郭小义, 戴云辉, 郭紫明, 等. 烟草科技), 2009(1): 43

[9] Fukushima R S, Hatfield R D. J Agric Food Chem, 2004, 52(12): 3713

[10] Cui P, Fang H X, Wu Q L, et al. Chinese Journal of Chromatography (崔朋, 方红霞, 吴强林, 等. 色谱), 2015, 33(3): 314

[11] Goni M A, Montgomery S. Anal Chem, 2000, 72(14): 3116

[12] Yu Y C, Li K L, Ma Y C, et al. Chinese Journal of Chromatography (于雅晨, 李坤兰, 马英冲, 等. 色谱), 2013, 31(2): 143

[13] Hedges J I, Ertel J R. Anal Chem, 1982, 54(2): 174

[14] Bahri H, Dignac M F, Rumpel C, et al. Soil Biol Biochem, 2006, 38(7): 1977

[15] Hautala K, Peuravuori J, Pihlaja K. Chemosphere, 1997, 35(4): 809

[16] Liu N, Zhang W, He H B, et al. Analytical Instrumentation (刘宁, 张威, 何红波, 等. 分析仪器), 2010(6): 30

[17] Steinberg S, Venkatesan M I, Kaplan I R. J Chromatogr, 1984, 298: 427

[18] GB 54136-2010

[19] Sun R, Lawther J M, Banks W B. Ind Crop Prod, 1995, 4(4): 241

[20] van der Hage E R E, Boon J J. J Chromatogr A, 1996, 736: 61