Determination of inorganic anions and organic acids in beer by replacement-ion chromatography with ultraviolet detection

doi:10.3724/SP.J.1123.2016.06008

Abstract

Abstract:

A new method for determination of inorganic anions and organic acids in beer was developed by replacement-ion chromatography-ultraviolet detection. Two ion replacement columns were introduced on the basis of the traditional ion chromatography. Inorganic anions and organic acids were converted into iodate salts with the same ultraviolet response, and then detected sensitively by ultraviolet detector instead of conductivity detector. The separation of the inorganic anions and organic acids was achieved on a Dionex AS11-HC anion chromatographic column with KOH as gradient eluent. The results showed that the limits of detection (S/N=3) and the limits of quantification (S/N=10) were 6.168-29.01 μg/L and 20.56-66.30 μg/L respectively; the linear relationship is good with correlation coefficients (r) greater than 0.9994. The average recoveries were between 89.0% and 117.0%, and relative standard deviations (RSDs) were all less than 1.0%, the detection results are almost the same with the results of traditional conductance. The method is simple, rapid and can realize the determination of inorganic ions and organic acids in beer using use a few standard curves.

Key words: beer, inorganic anions, organic acids, replacement-ion chromatography (RIC), ultraviolet detection

CLC Number:

O658

SHI Chaoou, YAO Baolong, HU Mi, CHEN Ailian. Determination of inorganic anions and organic acids in beer by replacement-ion chromatography with ultraviolet detection[J]. Chinese Journal of Chromatography, 2016, 34(10): 951-955.

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