Determination of olive oil content in olive blend oil by headspace gas chromatography-mass spectrometry

doi:10.3724/SP.J.1123.2017.02025

Abstract

Abstract:

A method for the determination of the content of olive oil in olive blend oil by headspace gas chromatography-mass spectrometry (SH-GC/MS) was established. The amount of the sample, the heating temperature, the heating time, the amount of injection, the injection mode and the chromatographic column were optimized. The characteristic compounds of olive oil were found by chemometric method. A sample of 1.0 g was placed in a 20 mL headspace flask, and heated at 180℃ for 2700 s. Then, 1.0 mL headspace gas was taken into the instrument. An HP-88 chromatographic column was used for the separation and the analysis was performed by GC/MS. The results showed that the linear range was 0-100%(olive oil content). The linear correlation coefficient (r²) was more than 0.995, and the limits of detection were 1.26%-2.13%. The deviations of olive oil contents in the olive blend oil were from -0.65% to 1.02%, with the relative deviations from -1.3% to 6.8% and the relative standard deviations from 1.18% to 4.26% (n=6). The method is simple, rapid, environment friendly, sensitive and accurate. It is suitable for the determination of the content of olive oil in olive blend oil.

Key words: blend oil, headspace gas chromatography-mass spectrometry (SH-GC/MS), olive oil

CLC Number:

O658

JIANG Wanfeng, ZHANG Ning, ZHANG Fengyan, YANG Zhao. Determination of olive oil content in olive blend oil by headspace gas chromatography-mass spectrometry[J]. Chinese Journal of Chromatography, 2017, 35(7): 760-765.

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