Rapid determination of 15 nutrients in rice by ultra high performance liquid chromatography-linear ion trap/orbitrap high resolution mass spectrometry

doi:10.3724/SP.J.1123.2017.05001

Abstract

Abstract:

A rapid method was developed for the simultaneous determination of 15 nutrients containing eight vitamin E, six γ-oryzanols and β-carotene in rice by ultra high performance liquid chromatography-linear ion trap/orbitrap high resolution mass spectrometry (UHPLC-LTQ/Orbitrap HRMS). The analytes were extracted by methanol containing 0.05% (v/v) 2,6-di-tert-butyl-4-methylphenol (BHT) under the ultrasonic condition, then separated by a Poroshell 120 PFP column (150 mm×3.0 mm, 2.7 μm) with a gradient elution program using 0.1% (v/v) formic acid aqueous solution and methanol containing 0.1% (v/v) formic acid as mobile phases. The detection was performed using an LTQ/Orbitrap HRMS detector with full scan in positive ion mode. Fifteen nutrients were simultaneously separated within 13 min. Furthermore, matrix effects of the white and brown rices were investigated. The correlation coefficients (r) were ≥ 0.9950 in the linear ranges of the 15 nutrients. The limits of detection (LODs, S/N=3) and limits of quantification (LOQs, S/N=10) of the 15 nutrients were 0.2-1.8 μg/L and 0.7-6.1 μg/L, respectively. At the three spiked levels, the recoveries were 73.2%-101.5% with the relative standard deviations (RSDs) ranging from 1.1% to 5.0% (n=3). The method is accurate, efficient and reliable. It is suitable for the simultaneous determination of various nutrients in rice.

Key words: β-carotene, γ-oryzanol, linear ion trap-orbitrap high resolution mass spectrometer (LTQ/Orbitrap HRMS), nutrients, rice, ultra high performance liquid chromatography (UHPLC), vitamin E

CLC Number:

O658

ZHU Li, TAN Wei, PENG Zumao, DENG Mengya, ZHANG Xieguang, YANG Guowu. Rapid determination of 15 nutrients in rice by ultra high performance liquid chromatography-linear ion trap/orbitrap high resolution mass spectrometry[J]. Chinese Journal of Chromatography, 2017, 35(9): 949-956.

References

[1] Thammapat P, Meesoaret N, Siriamornpun S. Food Chem, 2016, 194(5):230
[2] Qian L L, Song C L, Li Z J, et al. Food Science and Technology, 2015, 36(12):129 钱丽丽, 宋春蕾, 李志江, 等. 食品科技, 2015, 36(12):129
[3] Slavin J. Nutr Res Rev, 2004, 17(1):99
[4] Goufo P, Trindade H. Food Sci Nutr, 2014, 2(2):75
[5] Aladedunye F, Przybylski R, Rudzinska M, et al. J Am Oil Chem Soc, 2013, 90(8):1101
[6] Huang S H, Ng L T. J Chromatogr A, 2011, 1218(29):4709
[7] Allard J P, Aghdassi E, Chau J, et al. Aids, 1998, 12(13):1653
[8] The Parkinson Study Group. New Eng J Med, 1993, 328(3):176
[9] Nikiforova N V, Kirpatovsky V I, Darenkov A F, et al. Nephron, 1995, 69(4):449
[10] Azrina A, Maznah I, Azizah A H, et al. ASEAN Food J, 2008, 15(1):89
[11] Chen Y F, Jin J, Zheng S W, et al. Journal of the Chinese Cereals and Oils Association, 2014, 29(7):112 陈燕飞, 金俊, 郑苏卫, 等. 中国粮油学报, 2014, 29(7):112
[12] Stoggl W, Huck C, Wongyai S, et al. J Sep Sci, 2005, 28(14):1712
[13] Miller A, Frenzel T, Schmarr H G, et al. J Chromatogr A, 2003, 985(1/2):403
[14] Shammugasamy B, Ramakrishnan Y, Manan F, et al. Food Anal Methods, 2015, 8(3):649
[15] Fang N, Yu S G, Badger T M, et al. J Agric Food Chem, 2003, 51(11):3260
[16] Fan G X, Sun X W, Pan X R. Science and Technology of Cereals, Oils and Foods, 2010, 18(1):20 范国欣, 孙晓薇, 潘希荣. 粮油食品科技, 2010, 18(1):20
[17] Nielsen M M, Hansen A, et al. Cereal Chem, 2008, 85(2):248
[18] Kamal-Eldin A, Gorgen S, Pettersson J, et al. J Chromatogr A, 2000, 881(1/2):217
[19] Heinemann R J B, Xu Z M, Godber J S, et al. Cereal Chem, 2008, 85(2):243
[20] Ruperez F J, Martin D, Barbas H E, et al. J Chromatogr A, 2001, 935(1/2):45
[21] Zhu L, Yang S T, Li G K, et al. Anal Method, 2016, 8:5628
[22] Helfer A G, Michely J A, Weber A A, et al. Anal Chim Acta, 2015, 891(37):221
[23] Chen M H, Bergman C J. J Food Compos Anal, 2005, 18(4):319
[24] Matuszewski B K, Constanzer M L, Chavez-Eng C M. Anal Chem, 2003, 75(13):3019
[25] Farre M, Pico Y, Barcelo D. J Chromatogr A, 2014, 1328:66
[26] Liu Y Q, Li K, Xu W J, et al. Journal of Chinese Chromatography, 2016, 34(11):1055 刘永强, 李凯, 许文娟, 等. 色谱, 2016, 34(11):1055