Separation of alkaloids using radial chromatography and its separation efficiency

doi:10.3724/SP.J.1123.2018.05016

Abstract

Abstract:

The radial thin layer chromatography (RTLC) is a chromatographic method for the simple, rapid and efficient separation of samples from the center along the radial direction. Simple device was assembled to separate the alkaloids contented in Zhusha Anshen Pills (ZSASP). Based on the theory of thin layer chromatography, the test was designed to compare the separation efficiency of radial chromatography and general thin layer chromatography. The RTLC is faster, more efficient, more economical, and more suitable for the separation of high polar samples like alkaloids as proved. The theoretical root of the high separation efficiency of radial thin layer method was explored, which also provided new methods and ideas for theoretical innovation.

Key words: alkaloids, data fitting, plane chromatography, radial thin layer chromatography (RTLC), separation efficiency, test design verification

CLC Number:

O658

ZHANG Mengting, GONG Dandan, SUN Wanyang, SUN Guoxiang. Separation of alkaloids using radial chromatography and its separation efficiency[J]. Chinese Journal of Chromatography, 2018, 36(10): 1045-1052.

References

[1] Yin L, Zong L L, Pu X H, et al. Journal of Henan University (Medical Science), 2016, 35(2):77 尹丽, 宗兰兰, 蒲晓辉, 等. 河南大学学报(医学版), 2016, 35(2):77
[2] Ciura K, Dziomba S, Nowakowska J, et al. J Chromatogr A, 2017, 1520:9
[3] Zhu G L, Tong Q Y. Science and Technology of Food Industry, 2004, 25(1):131 朱桂兰, 童群义. 食品工业科技, 2004, 25(1):131
[4] Du L P, Shang J L, Wang D M, et al. Journal of Molecular Science, 2013, 29(1):84 杜莉萍, 商金玲, 王大明, 等. 分子科学学报, 2013, 29(1):84
[5] Wang Y, Li Y P, Xu L J, et al. Guangdong Chemical Industry, 2016, 43(13):32 王艳, 李艳萍, 徐丽娟, 等. 广东化工, 2016, 43(13):32
[6] Deng Y, Zhang J L, Wang L Y, et al. Chinese Journal of Pharmaceutical Analysis, 2018, 38(1):13 邓勇, 张杰良, 王兰英, 等. 药物分析杂志, 2018, 38(1):13
[7] Feng X L, Xie H, Liu X X, et al. West China Journal of Pharmaceutical Sciences, 2017, 32(4):439 冯霞兰, 谢华, 刘秀秀, 等. 华西药学杂志, 2017, 32(4):439
[8] Feng Y B, Du L, Wen J. Journal of Diseases Monitor & Control, 2011, 5(1):60 冯雅斌, 杜靓, 温静. 疾病监测与控制, 2011, 5(1):60
[9] Luo Y P, Deng P F. Journal of Anhui Agricultural Sciences, 2011, 39(6):3309 罗由萍, 邓鹏飞. 安徽农业科学, 2011, 39(6):3309
[10] Rokka J, Grönroos T J, Viljanen T, et al. J Chromatogr B, 2017, 1048:140
[11] Chen C, Cong Y Y, Remila M J T. Food Safety and Quality Detection Technology, 2017, 8(1):256 陈橙, 丛媛媛, 热米拉·米吉提. 食品安全质量检测学报, 2017, 8(1):256
[12] Zhang X G, Peng Z M, Zhang H, et al. China Oils and Fats, 2017, 42(7):35 张协光, 彭祖茂, 张涵, 等. 中国油脂, 2017, 42(7):35
[13] Tang H L, Yin H P. Food Industry, 2017, 38(1):195 唐惠玲, 尹鸿萍. 食品工业, 2017, 38(1):195
[14] Xu F, Feng Y, Fu S, et al. Chinese Journal of Hospital Pharmacy, 2016, 36(5):358 许凤, 冯钰, 付双, 等. 中国医院药学杂志, 2016, 36(5):358
[15] Chen Y Q, Hao Y, Qi X Y, et al. Research and Practice on Chinese Medicines, 2016, 30(2):10 陈艳琼, 郝勇, 戚雪勇. 现代中药研究与实践, 2016, 30(2):10
[16] Li Q Y, Wu Q. China Petroleum and Chemical Standard and Quality, 2012, 33(15):23 李晴媛, 吴青. 中国石油和化工标准与质量, 2012, 33(15):23
[17] Vitek R K, Waltking A E, Kent D M. Liq Chromatogr Anal F & B, 1979, 33:215
[18] Lynam C G, Weil H. Manu Chem & A N, 1950, 21(5):195
[19] Zhang S N, Shi J, Zhang S J. Journal of Tianjin Agricultural University, 2008, 15(1):38 张淑娜, 石军, 张淑娟. 天津农学院学报, 2008, 15(1):38
[20] KramLova M, Pristoupil T I. J Chromatogr, 1968, 7(2):264
[21] Miller R B, Case W S. J Chem Edu, 2011, 88(9):1328
[22] Zhou T H. Paper Chromatography and Thin-Layer Chromatography. 3rd ed. Beijing:Science Press, 1989 周同惠. 纸色谱和薄层色谱. 3版. 北京:科学出版社, 1989
[23] Zhang G P, Liang Y S. China Health Care Innovation, 2008, 3(10):99 张广萍, 梁延寿. 中国医疗前沿, 2008, 3(10):99
[24] Yin X, Sun G X. Central South Pharmacy, 2011, 9(2):135 尹霞, 孙国祥. 中南药学, 2011, 9(2):135
[25] Sun Y Q. Chinese Journal of Chromatography, 2008, 26(3):342 孙毓庆. 色谱, 2008, 26(3):342
[26] Guo L G, Sun S, Xie P S, et al. Traditional Chinese Drug Research and Clinical Pharmacology, 2015, 26(1):92 郭隆钢, 孙帅, 谢培山, 等. 中药新药与临床药理, 2015, 26(1):92
[27] Sun J B, Wang X, Qin Y, et al. West China Journal of Pharmaceutical Sciences, 2014, 29(6):675 孙建彬, 王欣, 覃瑶, 等. 华西药学杂志, 2014, 29(6):675
[28] Gu Z R, Chen H, Wang Y L, et al. Lishizhen Medicine and Materia Medica Research, 2014, 25(9):2164 顾志荣, 陈晖, 王亚丽, 等. 时珍国医国药, 2014, 25(9):2164
[29] Kong F, Ding Y, Liang X, et al. Acta Chinese Medicine and Pharmacology, 2013, 41(2):56 孔菲, 丁莹, 梁雪, 等. 中医药学报, 2013, 41(2):56
[30] Wang J.[MS Dissertation]. Shijiazhuang:Hebei Normal University, 2013 王静.[硕士学位论文]. 石家庄:河北师范大学, 2013
[31] Li F M. Analytical Chemistry. 7th ed. Beijing:People's Medical Publishing House, 2014 李发美. 分析化学. 7版. 北京:人民卫生出版社, 2014
[32] Sun Y Q. Optimization and Selection of Liquid Chromatography Solvent System. Beijing:Chemical Industry Press, 2008 孙毓庆. 液相色谱系统的优化与选择. 北京:高等教育出版社, 2008
[33] Zhao Z J. Chinese Journal of Analytical Chemistry, 1983, 11(5):390 赵中杰. 分析化学, 1983, 11(5):390