Abstract: An isotope dilution liquid chromatographic-electrospray ionization tandem mass spectrometric method has been established for the determination of acrylamide in heated starchy food with a C18 analytical column and methanol-water containing 0.1% formic acid (2∶98, v/v) as mobile phases. The clean-up of samples was performed on an Oasis HLB solid phase extraction cartridge. The calibration curve of acrylamide showed good linearity in the range of 10-500 μg/L with correlation coefficient of 0.9995. The detection limit of the method was 6 μg/kg (S/N=3), and the limit of quantification was 20 μg/kg (S/N=10). The average recoveries at three levels ranged from 96.8% to 97.4% (n=5), and the relative standard derivations were lower than 10%.

Key words: , acrylamide, liquid chromatography-electrospray ionization tandem mass spectrometry