Chinese Journal of Chromatography

Chinese Journal of Chromatography

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Determination of α-Linolenic Acid in Perilla Oil by Reversed-Phase High Performance Liquid Chromatography Coupled with Evaporative Light-Scattering Detector

WANG Qiao’e1, SONG Ping2, DING Mingyu1   

  1. 1.Key Lab of Bioorganic Phosphorous Chemistry &Chemical Biology, Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, China; 2.Department of Chemistry, Qinghai Nationalities Institute, Xining 810007, China
  • Received:2005-02-21 Revised:2005-11-21 Online:2006-01-30 Published:1987-09-25

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Abstract:

A method for the determination of α-linolenic acid in perilla oil was developed using the reversed-phase high performance liquid chromatography coupled with evaporative light-scattering detector (RP-HPLC-ELSD). The perilla oil was saponified by 0.5 mol/L KOH-CH3OH solution for 20 min in a 60 ℃ water bath, then acidified by 6 mol/L HCl and finally the dissociative fatty acids, including α-linolenic acid, was extracted by anhydrous ether. After the ether was blown out by nitrogen, the residuals were dissolved by 10 mL methanol. The calibration curve was found to be linear over the range of 6.2-45.4 μg (r=0.9973, n＝5) and the detection limit was 0.11 μg (S/N=3). The average recovery was 102% and their relative standard deviation (RSD) was 6.3% (n＝5). The content of α-linolenic acid in the determined perilla oil was 6.79% which is consistent with the previous report.

Key words: evaporative light-scattering detector (ELSD), perilla oil , α-linolenic acid, reversed-phase high performance liquid chromatography (RP-HPLC)