Chinese Journal of Chromatography

Chinese Journal of Chromatography

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Determination of Aflatoxins in Hot Chilli Products by Matrix Solid-Phase Dispersion and Liquid Chromatography

ZHENG Ping1, SHENG Xuan1, YU Xiaofeng1, HU Yanyun2   

  1. 1.Anhui Entry-Exit Inspection and Quarantine Bureau, Hefei 230061, China; 2.Department of Chemistry, University of Science and Technology of China, Hefei 230026, China
  • Received:2005-01-13 Revised:2005-06-16 Online:2006-01-30 Published:1987-09-25

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Abstract:

A new method based on matrix solid-phase dispersion (MSPD) extraction with neutral alumina and co-column purification with graphitized carbon black has been developed to determine aflatoxins B1, B2, G1, G2 in hot chilli products. The method includes liquid chromatography and fluorescence detection with on-line post-column derivatization with bromine. Optimization of different parameters, such as the type of solid supports for matrix dispersion and co-column clean-up was carried out. The recoveries of aflatoxins B1, B2, G1 and G2 were 95.4%, 87.3%, 91.5% and 92.6%, respectively, with relative standard deviations ranging from 3.3% to 6.1%. The limits of detection were in the range of 0.10 ng/g (B2, G2) to 0.25 ng/g (B1, G1). In addition, the comparison of the extraction and purification effect of MSPD with immunity affinity column showed that, MSPD is a valid method to analyze aflatoxins in hot chilli products.

Key words: aflatoxins, hot chilli products , liquid chromatography (LC), matrix solid-phase dispersion (MSPD)