Chinese Journal of Chromatography

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Extraction and Determination of Essential Oils in Indocalamus
latifolius Leaves and Indocalamus tessellatus Leaves

LI Shuifang1, WEN Ruizhi1, ZENG Dong2, LI Zhonghai3   

  1. 1.College of Science, Central South University of Forestry &Technology, Zhuzhou 412006, China;
    2.Hunan Center for Disease Control and Prevention, Changsha 410005, China;
    3.College of Life Science and Technology, Central South University of
    Forestry &Technology, Changsha 410004, China
  • Received:2006-03-20 Revised:2006-06-30 Online:2007-01-30 Published:1986-03-25

Abstract:

Indocalamus leaves are the generic name of the leaves of Indocalamus (Graminales) plants. The essential oils in Indocalamus latifolius leaves and Indocalamus tessellatus leaves were extracted by steam distillation method. Ether was used as the solvent to extract volatile compounds many times. Both the volatile compounds in Indocalamus latifolius leaves and Indocalamus tessellatus leaves were analyzed by gas chromatography-mass spectrometry (GC-MS). The results showed that thirty-seven compounds were identified for the essential oils extracted from Indocalamus latifolius leaves, and its main components were found to be 3-hexen-1-ol, (Z)-; 1-hexanol; benzyl alcohol; hexanal; furan, 2-ethyl-; 3-buten-2-one, 4-(2,6,6-trimethyl-1-cyclohexen-1-yl), (E)-; etc. Forty-nine components were identified for the essential oils extracted from Indocalamus tessellatus leaves, and its main components were found to be 3-hexen-1-ol, (Z)-; benzyl alcohol; 3-buten-2-one, 4-(2,6,6-trimethyl-1-cyclohexen-1-yl)-, (E)-; 2-hexenal; phenylethyl alcohol, 2-methoxy-4-vinylphenol; furan, 2-ethyl-; etc. 3-Hexen-1-ol, (Z)- was the most abundant compound in the essential oils from both Indocalamus leaves. There were ketone, aldehyde, alcohol, phenol and ester in them. The contents of ketones, aldehydes, and alcohols were found higher than those of other compounds in the two essential oils.

Key words: essential , Indocalamus latifolius leaves, Indocalamus tessellatus leaves, gas chromatography-mass spectrometry (GC-MS)