Abstract: A novel mimetic biomembrane chromatographic stationary phase of magnesia-zirconia composite matrix was prepared based on the Lewis acid-base interaction between the phosphonate group of phosphatidylcholine residue and the Lewis acid sites of magnesia-zirconia composite. The infrared absorption spectrum and X-ray photoelectron spectrum of the stationary phase illustrated that the magnesia-zirconia composite was successfully modified with phosphatidylcholine. The interactions between the membrane and the drugs were evaluated. It is observed that the log Kmbm values have good relationships with the log Papp, and the linear slope is 1.049, which is near unity. Moreover, on the basis of the thermodynamics derivation, the difference in standard free energies （Δ(ΔG°)） is introduced to describe the drug-membrane interaction. The results show that the log Kmbm and Δ (ΔG°) value provide key information on the transport properties of the drugs. The establishment of this chromatographic model may be a new way for the evaluation of the drug-membrane interactions.

Key words: apparent permeability (Papp), drug-membrane interaction , magnesia-zirconia composite, mimetic biomembrane stationary phase, liquid chromatography(LC)