Chinese Journal of Chromatography

Chinese Journal of Chromatography

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Classification of the cell lines in the extraction of intracellular metabolites based on ultra performance liquid chromatography-time of flight mass spectrometry

LI Hui1,2, YU Zhiguo2*, ZU Xuyu1, LIU Feng1, JIN Yibao1,2, JIANG Yuyang1,3, LIU Hongxia1*   

  1. 1.Key Laboratory of Chemical Biology of Guangdong Province, Graduate School of Shenzhen, Tsinghua University, Shenzhen 518055, China; 2.School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China; 3.School of Medicine, Tsinghua University, Beijing 100084, China
  • Received:2009-04-27 Revised:2009-06-26 Online:2009-07-30 Published:1982-06-25
  • Contact: YU Zhiguo，LIU Hongxia

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Abstract: Intracellular metabolites can reflect the physiological state of cells. Ultra performance liquid chromatography/time of flight mass spectrometry (UPLC-TOF MS) is a relatively new technique for the separation of complex samples. The aim of this work is to assess the feasibility of metabonomics in cell line sorting. In the work, a total of 5 cell line samples were analyzed by using UPLC-TOF MS and small molecules metabolite profiles from the extraction of intracellular metabolites were obtained. Principal component analysis (PCA) models were used to extract meaningful information from the complex biological samples. The cell line discrimination was highly improved by PCA. These preliminary results suggested that UPLC/MS coupled with pattern recognition show promise for metabonomics. It is a potential and very promising technology for the classification of the cell lines.

Key words: cell line sorting, metabonomics, principal component analysis (PCA) , ultra performance liquid chromatography/time of flight mass spectrometry (UPLC-TOF MS)