Abstract:

The abundance of glycoproteins and the glycans attached are usually low in body. In order to analyze them accurately and eliminate the interference of non-glycoproteins and non-glycopeptides, methods of enrichment are needed. A kind of novel magnetic nanoparticles based on click reaction and multiple-functional-site poly-(allyl chloride) structure is designed and prepared. The related hydrophilic enrichment method for glycopeptide is developed. Totally 32 glycopeptide signals were detected from human immunoglobulin G trypsin enzymolysis under the optimized conditions. The method can reach a limit of detection of 10 fmol on human immunoglobulin G trypsin enzymolysis, with an adsorption capacity of 100 mg of 1 g particles.

Key words: click reaction, glycopeptide, human immunoglobulin G, magnetic enrichment nanomaterials