Teaching practice of undergraduate graduation projects in clinical laboratory science integrating liquid chromatography-tandem mass spectrometry and external quality assessment

doi:10.3724/SP.J.1123.2025.06030

Abstract

Abstract:

This study aimed to explore the application and pedagogical innovation of liquid chromatography-tandem mass spectrometry （LC-MS/MS） in undergraduate thesis training for medical laboratory science students. Using the development of 25-hydroxyvitamin D₂ （25（OH）D₂） and 25-hydroxyvitamin D₃ （25（OH）D₃） external quality assessment （EQA） samples as a teaching platform， an experimental teaching model driven by research tasks and clinical practice was established. The teaching design encompassed the entire workflow， including sample preparation， instrument operation， data processing， and result evaluation， enabling students to acquire hands-on experience with core LC-MS/MS techniques and quality control procedures. Through the implementation of the experimental project， students participated in EQA sample preparation， reference method-based quantification， homogeneity and stability assessments， and EQA result analysis， thereby gaining comprehensive exposure to the process from experimental design to result interpretation. The results demonstrated that the prepared EQA samples exhibited satisfactory homogeneity and stability， meeting the quality standards established by the China National Accreditation Service for Conformity Assessment （CNAS）. In the “2024 Beijing Serum Vitamin D EQA” activity， a total of 16 medical laboratory institutions in Beijing participated. Teaching practice revealed that this project significantly enhanced students’ comprehensive abilities in experimental design， instrument operation， data analysis， and quality assessment， while also fostering scientific thinking and teamwork. By introducing advanced analytical techniques into the undergraduate curriculum under the educational concept of “research-driven teaching and integration of industry and education”， this approach not only optimized course content and experimental procedures but also broadened students’ practical perspectives in clinical laboratory and research applications. This study provides a replicable teaching model for undergraduate laboratory education and offers valuable insights for promoting the integration of advanced analytical technologies into medical laboratory science training.

Key words: liquid chromatography-tandem mass spectrometry（LC-MS/MS）, 25-hydroxyvitamin D₂（25（OH）D₂）, 25-hydroxyvitamin D₃（25（OH）D₃）, external quality assessment （EQA）, clinical laboratory science, teaching innovation

CLC Number:

O658

SHI Jie, WANG Mo, XUE Qiaozhen, ZHANG Shunli, HU Yanwei. Teaching practice of undergraduate graduation projects in clinical laboratory science integrating liquid chromatography-tandem mass spectrometry and external quality assessment[J]. Chinese Journal of Chromatography, 2026, 44(3): 363-370.

Figures/Tables 4

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Batch No.	Source of variation	Degree of freedom	Sum of squares	Mean square	F
202411	between the samples	9	0.518	0.058	1.78
202411	within the sample	10	0.323	0.032	1.78
202412	between the samples	9	24.875	2.764	1.90
202412	within the sample	10	14.475	1.448	1.90

Batch No.	Source of variation	Degree of freedom	Sum of squares	Mean square	F
202411	between the samples	9	0.518	0.058	1.78
202411	within the sample	10	0.323	0.032	1.78
202412	between the samples	9	24.875	2.764	1.90
202412	within the sample	10	14.475	1.448	1.90

Batch No.	Source of variation	Degree of freedom	Sum of squares	Mean square	F
202411	between the samples	9	5.769	0.641	2.46
202411	within the sample	10	2.609	0.261	2.46
202412	between the samples	9	113.025	12.558	1.08
202412	within the sample	10	116.374	11.637	1.08

Batch No.	Source of variation	Degree of freedom	Sum of squares	Mean square	F
202411	between the samples	9	5.769	0.641	2.46
202411	within the sample	10	2.609	0.261	2.46
202412	between the samples	9	113.025	12.558	1.08
202412	within the sample	10	116.374	11.637	1.08

Project name	Batch No.	Target value/（nmol/L）	Acceptable lower limit/（nmol/L）	Acceptable upper limit/（nmol/L）
25（OH）D₂	202411	21.30	15.98	26.63
	202412	10.30	7.73	12.88
	202413	6.42	4.82	8.03
25（OH）D₃	202411	17.05	12.79	21.31
	202412	63.00	47.25	78.75
	202413	15.11	11.33	18.89
Total 25（OH）D	202411	38.35	28.76	47.94
	202412	73.30	54.98	91.63
	202413	21.53	16.15	26.91