Abstract:

This study analyzed the effect of Sargassum fusiforme polysaccharides (SFPS) on the complex mobility of erythrocytes in tumor-bearing organisms. A mouse tumor model was developed in which mice were intraperitoneally injected with high, medium, and low dosages of SFPS for 7 d. The erythrocytes were collected and prepared into suspensions, and the complex mobilities of cells were measured using high performance capillary electrophoresis (HPCE). Experimental conditions included the following: capillaries, 75 μm×50 cm; buffer for electrophoresis, phosphate solution containing hydroxypropylmethyl cellulose (0.1 mol/L, pH 7.4); injection pressure, 3.448 kPa; injection time, 10 s; separation voltage, 20 kV; column temperature, 25 ℃. The electroosmotic mobility was calculated to be 2.16×10-4 cm2·V-1·s-1. It was found that SFPS can decrease the migration time of erythrocytes in tumor-bearing organisms, which was 17.56 min for the control group, 13.96 min for the low dosage group, 12.90 min for the medium dosage group, 13.51 min for the high dosage group, and 11.51 min for the normal group, and increase its complex mobility, which was 1.06×10-4 cm2·V-1·s-1 for the control group, 1.19×10-4 cm2·V-1·s-1 for the low dosage group, 1.29×10-4 cm2·V-1·s-1 for the medium dosage group, 1.23×10-4 cm2·V-1·s-1 for the high dosage group, and 1.45×10-4 cm2·V-1·s-1 for the normal group. Compared with the negative control group, all three dosage groups had significant differences (P＜0.01) in the complex mobility of erythrocytes. It was found that SFPS can change the complex mobility of erythrocytes in tumor-bearing organisms with the tendency to the condition in normal organisms, which is possibly because SFPS can change the charges density on erythrocytes surface. It is believed that HPCE can be used as an auxiliary tool for determining the physiological state and functions of erythrocytes.

Key words: complex mobility, erythrocyte, Sargassum fusiforme
polysaccharides, tumor organism , high performance capillary electrophoresis (HPCE)