Abstract:

A simple reversed-phase high performance liquid chromatographic method was developed for the determination of dencichine in sanchi on a porous graphitic carbon (PGC) column. The effects of different buffers, buffer concentrations, pH values of mobile phase and mobile phase compositions as well as column temperatures on the retention of dencichine were studied. The retention mechanism of dencichine on a porous graphitic carbon column seems to be mainly hydrophobic interaction according to the study. The chromatographic method developed was used to determine dencichine in sanchi. The calibration curve was linear in the range of about 0.22-4.4 μg (r=0.9999) and the recovery for the extract was 99.5% with the relative standard deviation of 2.2% (n=9).

Key words: dencichine, retention mechanism, sanchi , porous graphitic carbon column