Abstract:

The chromatographic behavior of purine derivatives on a new cucurbit［6］uril monorotaxane-bonded silica stationary phase (CB6MRBS) was studied using high performance liquid chromatography both under normal and reversed-phase modes. A comparative study of this phase with ODS was carried out as well. The influences of methanol content, pH value and ionic strength of mobile phases were investigated in detail. The results showed that various interactions occurred between the analytes and CB6MRBS besides hydrophobic interaction under reversed-phase were different from those on ODS. According to the chromatographic analysis, the separation mechanism of multiple interactions also exists under normal phase. The CB6MRBS can provide various sites for analytes, such as the hydrophobic, hydrogen-bonding, π-π and dipole-dipole interactions. Separations of purine derivatives on CB6MRBS were achieved with satisfaction due to the various retention mechanisms both under reversed- and normal phase modes.

Key words: cucurbit［6］uril monorotaxane-bonded silica stationary phase, purine derivatives , separation mechanism, high performance liquid chromatography (HPLC)