Chinese Journal of Chromatography

Chinese Journal of Chromatography

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Preparation of three capsaicinoid components using preparative high performance liquid chromatography

DONG Xinrong1,2, LIU Zhonghua2, LI Benxiang1, WANG Kunbo2   

  1. 1.College of Science, Hunan Agricultural University, Changsha 410128, China;
    2.Natural Product Research Center, Hunan Agricultural University, Changsha 410128, China
  • Received:2007-08-24 Revised:2007-10-19 Online:2008-05-30 Published:1984-03-25
  • Contact: LIU Zhonghua

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Abstract:

A method was established for the isolation of three capsaicinoid components from capsaicinoids using
preparative high performance liquid chromatography (Pre-HPLC). A preparative PRC-ODS column (250 mm×21.5 mm, 13
μm) was used in the preparation process. By isocratic elution with methanol-water (70∶30, v/v) as the mobile phase
at a flow rate of 15 mL/min followed by concentration, three capsaicinoid components were obtained from 80%
capsaicinoids, and subsequently identified by 1H NMR （nuclear magnetic resonance） and EI-MS （electron impact-mass
spectrometry） as nordihydrocapsaicin, capsaicin and dihydrocapsaicin, and the purities of the three components
analyzed by HPLC were 98.12%, 99.93% and 100.7% with yields of 60.1%, 58.9% and 72.3%, respectively.

Key words:
dihydrocapsaicin, capsaicin, capsaicinoids , nordihydrocapsaicin, preparative high performance liquid chromatography （Pre-HPLC）