Abstract:

Triazolam is often abused in criminal activities. It is necessary to develop a simple, fast and sensitive
method for determining triazolam in human urine. After the triazolam in urine was extracted with ether at pH 9, the
analysis was performed on an HP-5 capillary column with gas chromatograph-micro electron capture detector (GC-
μECD). The calibration curve of triazolam showed good linearity in the range of 0.2-50 ng/mL with the correlation
coefficient of 0.9995. The inter-day relative standard deviation (RSD) and intra-day RSD were 4.17% and 5.31%,
respectively. The average recovery was 93.9%. This simple, sensitive and accurate method can be easily implemented
for monitoring triazolam in urine.

Key words: human urine , micro electron capture detector (μECD), triazolam, gas chromatography (GC)