Abstract: The two pairs of enantiomers of epoxiconazole were resolved using normal phase, reversed-phase and polar organic solvent high performance liquid chromatography on cellulose tris(3,5-dimethylphenylcarbamate) (CDMPC) chiral stationary phase, separately. The effects of different mobile phase compositions on the retention factor, separation factor and resolution in the chiral separation of epoxiconazole were investigated. It can be baseline separated on a Chiralcel OD-H column packed with CDMPC chiral stationary phase when methanol-water (80∶20, v/v) was used as the mobile phase, the resolutions of the two pairs of enantiomers were 1.64 and 6.50, respectively.

Key words: enantioseparation, normal phase liquid chromatography （NPLC）, polar organic solvent liquid chromatography, racemic epoxiconazole , reversed-phase liquid chromatography （RPLC）, cellulose tris (3,5-dimethylphenylcarbamate) chiral stationary phase