Optimization of experimental conditions for the separation of polystyrene particles by gravitational field-flow fractionation

doi:10.3724/SP.J.1123.2016.07020

Abstract

Abstract:

Gravitational flow-field fractionation (GrFFF) is the simplest and cheapest field-flow fractionation technique. GrFFF utilizes earth's gravity as external field, providing a size-based separation. It can be applied to the separation and characterization of various micrometer-sized particles and biological samples. A GrFFF device was assembled, and three kinds of polystyrene (PS) particles with different sizes (20, 6 and 3 μm) were used as the model particles. A homemade mixed surfactant was used and was compared with the surfactant FL-70. The separation conditions of GrFFF were optimized with uniform design, including flow rate, mass percentage of polyethylene glycol octylphenol ether (Triton X-100) in mixed surfactant, viscosity of carrier liquid and stop-flow time. The resolution (R_s) and retention ratio (R) were utilized as the evaluation indexes. The results suggest that the separation performance of FL-70 was slightly better than that of the mixed surfactant. Using FL-70 as the surfactant, particles of 20, 6 and 3 μm were completely separated (with resolutions of 1.771 and 2.074). The results indicate that this system has good separation performance.

Key words: carrier liquid, flow rate, gravitational field-flow fractionation(GrFFF), polystyrene (PS) particles, resolution, surfactant, uniform design

CLC Number:

O658

QIU Bailing, WU Di, GUO Shuang, ZHU Chenqi, GAOYANG Yaya, LIANG Qihui, GAO Ye, SONG Yu, HAN Nanyin. Optimization of experimental conditions for the separation of polystyrene particles by gravitational field-flow fractionation[J]. Chinese Journal of Chromatography, 2017, 35(2): 216-221.

References

[1] Giddings J C. Sep Sci, 1966, 1:123
[2] Schachermeyer S, Ashby J, Zhong W W. Anal Bioanal Chem, 2012, 404:1151
[3] Quan C, Liu P P, Jin J S. Chemistry, 2013, 76(1):52全灿, 刘攀攀, 金君素. 化学通报, 2013, 76(1):52
[4] Park M R, Kang D K, Chmelik J, et al. J Chromatogr A, 2008, 1209:206
[5] Plockva J, Chmelik J. J Chromatogr A, 2001, 918:361
[6] Plockva J, Chmelik J. J Chromatogr A, 2006, 1118:253
[7] Lee S, Kang D Y, Park M, et al. Anal Chem, 2011, 83:3343
[8] Reschiglian P, Melucci D, Torsi G. J Chromatogr A, 1996, 740:245
[9] Jiang D L, Shao L Y. Journal of Jilin Normal University:Natural Science Edition, 2009, 30(1):78蒋东霖, 邵丽颖. 吉林师范大学学报:自然科学版, 2009, 30(1):78
[10] Plockva J, Matulik F, Chmelik J. J Chromatogr A, 2002, 955:95
[11] Shao L Y, Jiang D L. Journal of Jilin Normal University:Natural Science Edition, 2011, 32(1):91邵丽颖, 蒋东霖. 吉林师范大学学报:自然科学版, 2011, 32(1):91
[12] Lattuade D, Roda B, Pignatari C, et al. Anal Bioanal Chem, 2013, 405:977
[13] Roda B, Casolari S, Reschiglian P, et al. Anal Bioanal Chem, 2009, 394:953
[14] Casolari S, Roda B, Mirasoli M, et al. Analyst, 2013, 138:211
[15] Guo S, Zhu C Q, Gaoyang Y Y, et al. Chinese Journal of Chromatography, 2016, 34(2):146郭爽, 朱尘琪, 高杨亚雅, 等. 色谱, 2016, 34(2):146
[16] Zhang X J.[PhD Dissertation]. Changchun:Jilin University, 2007张学军.[博士学位论文]. 长春:吉林大学, 2007
[17] Woo I S, Jung E C, Lee S. Talanta, 2015, 132:945
[18] Cen G Q.[MS Dissertation]. Haikou:Hainan University, 2012岑桂秋.[硕士学位论文]. 海口:海南大学, 2012
[19] Shao L Y, Jiang D L, Lü Y J. Journal of Yanbian University:Natural Science, 2008, 34(4):258邵丽颖, 蒋东霖, 吕英军. 延边大学学报:自然科学版, 2008, 34(4):258.