Determination of 10 volatile N-nitrosamines in meat products by gas chromatography-tandem mass spectrometry

doi:10.3724/SP.J.1123.2018.09037

Abstract

Abstract:

A gas chromatography-tandem mass spectrometry (GC-MS/MS) method was established for the determination of 10 volatile N-nitrosamines in meat products. The meat samples were extracted by simultaneous distillation extraction (SDE), and then a cleanup step involving the frozen fat removal method was applied. The analytes were quantified by the external standard method in multiple reaction monitoring (MRM) mode. Under the optimized conditions, the correlation coefficients of the standard calibration curves were greater than 0.99 in the range of 1.00-1000 μg/L. The limits of detection (LODs, S/N=3) and limits of quantification (LOQs, S/N=10) were 0.01-0.02 μg/kg and 0.04-0.07 μg/kg, respectively. The average spiked recoveries of the 10 volatile N-nitrosamines were 74.8%-94.3% at spiked levels of LOQ level, 1.0 and 2.0 μg/kg,and the relative standard deviations were less than 8.3%. Six volatile N-nitrosamines (N-nitrosodimethylamine (NDMA), N-nitrosodiethylamine (NDEA), N-nitrosopyrrolidine (NPYR), N-nitrosopiperidine (NPIP), N-nitrosodibutylamine (NDBA) and N-nitrosodi-n-propylamine (NDPA)) were detected in different types of meat products, and each volatile N-nitrosamine in pickled meat products had the highest detection values. The developed method has the advantages of simplicity, sufficient extraction, high sensitivity, and low reagent dosage, in addition to proving suitable for the daily testing requirements of a large number of samples in the laboratory.

Key words: N-nitrosamines, freezing lipid filtration, gas chromatography-tandem mass spectrometry (GC-MS/MS), meat products, simultaneous distillation extraction (SDE)

CLC Number:

O658

ZHU Mengmeng, YE Qun, ZHOU Tingting, CHEN Lutao, YU Luping, LI Bin, HU Jinfeng, ZHOU Min. Determination of 10 volatile N-nitrosamines in meat products by gas chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2019, 37(2): 207-215.

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