Nano Flow Multidimensional Liquid Chromatography for Proteome Analysis of Rat Liver

Chinese Journal of Chromatography

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Nano Flow Multidimensional Liquid Chromatography for Proteome Analysis of Rat Liver

WANG Yan, GAO Mingxia, GU Xue, ZHANG Xiangmin

Department of Chemistry, Fudan University, Shanghai 200032, China

Received:2004-06-23 Revised:1900-01-01 Online:2005-01-30 Published:1989-03-25

Abstract

Abstract: A nano flow comprehensive multidimensional liquid chromatography was developed. By using this separation platform, complex samples can be injected, desalted, separated and analyzed in complete automatization. Coupling of the strong cation-exchange (SCX) with capillary reversed-phase liquid chromatography (cRPLC) is described for proteomics of rat liver. A step gradient was applied for SCX column, and a linear gradient was applied for RPLC. The flow rate through the nano scale analytical column during the separation process was maintained at 200 nL/min. The peak capacity for the nano-2D-LC was up to 620 under these conditions.

Key words: multidimensional liquid chromatography, nano flow , rat liver, proteomics

WANG Yan, GAO Mingxia, GU Xue, ZHANG Xiangmin . Nano Flow Multidimensional Liquid Chromatography for Proteome Analysis of Rat Liver[J]. Chinese Journal of Chromatography.

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Nano Flow Multidimensional Liquid Chromatography for Proteome Analysis of Rat Liver

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