Chinese Journal of Chromatography

Chinese Journal of Chromatography

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Analysis of Recombinant Erythropoietin and Its Tryptic Digest by Capillary Electrophoresis and Capillary Electrophoresis-Electrospray Ionization-Mass Spectrometry Using Capillary Coated with 6,6-Ionene

YU Bing, CONG Hailin, LIU Huwei, LI Yuanzong, LIU Feng
  

  1. The Key Lab of Bioorganic Chemistry and Molecular Engineering of Ministry of Education,
    College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
  • Received:2005-05-08 Revised:1900-01-01 Online:2005-09-30 Published:1988-03-25

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Abstract:

The microheterogeneity of recombinant human erythropoietin (rhEPO) was analyzed by capillary electrophoresis (CE) using a capillary coated with 6,6-ionene. The applicability of a volatile electrolyte for fast analysis of tryptic fragments of rhEPO with online CE-electrospray ionization-mass spectrometry (ESI-MS) was investigated, resulting in a reproducible separation of eleven rhEPO tryptic fragments within 22 min under the following conditions: running buffer 300 mmol/L acetic acid-ammonium acetate (HAc-NH4Ac), pH 4.80, separation voltage -15 kV and capillary temperature 25 ℃. The proposed method is rapid and effective, and can be used for the structural analysis of related proteins.

Key words: microheterogeneity, recombinant human erythropoietin, tryptic digest, 6,6-ionene coating, capillary electrophoresis coupled with electrospray ionization-mass spectrometry, tryptic mapping , capillary electrophoresis