Abstract:

The capillary electrophoresis fingerprints (CEFP) of Fructus Forsythiae was established to control its quality. The background electrolyte (BGE) was a 75 mmol/L sodium borate solution adjusted to pH 9.7 with 0.1 mmol/L NaOH solution. The detection wavelength was 228 nm and a voltage of 14 kV was applied. The Forsythia suspensa (Thunb.) Vahl was extracted by water and a set of CEFP containing 29 peaks was obtained. The CEFP had acceptable precision and reproducibility with the relative standard deviation (RSD) of the relative migration time less than 5.0% and the RSD of the relative peak area from 2% to 15%. There were good similarities between the standard CEFP and each set of CEFP of Forsythia suspensa (Thunb.) Vahl from ten different places, and their similarity coefficients were not less than 0.94. The CEFP were also evaluated by the information index I and the relative information index Ir of chromatographic fingerprint.

Key words: fingerprints, Fructus Forsythiae, information index, relative information index , capillary electrophoresis(CE)