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Chinese Journal of Chromatography
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Chinese Journal of Chromatography  2015, Vol. 33 Issue (6): 642-646    DOI: 10.3724/SP.J.1123.2015.01026
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Comparison among three anion exchange chromatographic supports to capture erythropoietin from cell culture supernatant Lourdes
Lourdes HERNÁNDEZ1,2, Diobel STEWART1, Lourdes ZUMALACÁRREGUI2, Daniel AMARO1,2
1. Development Product Department, Manufacturing Plant of Recombinant Proteins and Vaccines, Center for Molecular Immunology, Havana 11600, Cuba;
2. Department of Chemical Engineering, Faculty of Chemical Engineering, Higher Polytechnic Institute José Antonio Echeverría, Havana 19390, Cuba
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Abstract 

Affinity and ion exchange conventional chromatography have been used to capture erythropoietin (EPO) from mammalian cell culture supernatant. Currently, chromatographic adsorbent perfusion is available, however a limited number of applications have been found in the literature. In this work, three anion exchange chromatographic supports (gel, membrane and monolithic) were evaluated in the capture step of the recombinant erythropoietin purification process. The influences of load and flow rate on each support performance were analyzed. Also the purity of the EPO molecules was determined. A productivity analysis, as a decision tool for larger scale implementation, was done. As a conclusion, the evaluated supports are technically suitable to capture EPO with adequate recovery and good purity. However, the monolithic column admits high operating velocity, showing the highest adsorption capacity and productivity.

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Articles by authors
Lourdes HERNÁ
NDEZ
Diobel STEWART
Lourdes ZUMALACÁ
RREGUI
Daniel AMARO
Key wordsanion exchange chromatography   supports   recombinant erythropoietin   purification     
Received: 2015-01-22;
Cite this article:   
Lourdes HERNÁ,NDEZ,Diobel STEWART et al. Comparison among three anion exchange chromatographic supports to capture erythropoietin from cell culture supernatant Lourdes[J]. Chinese Journal of Chromatography, 2015, 33(6): 642-646.
 
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[1] André Moreni LOPES1*, Jorge Sánchez ROMEU2#, Rolando Páez MEIRELES2, Gabriel Marquez PERERA2, Rolando Perdomo MORALES3, Adalberto PESSOA Jr1, Lourdes Zumalacárregui CáRDENAS4. Adsorption of endotoxins on Ca2+ iminodiacetic acid by metal ion affinity chromatography[J]. Chinese Journal of Chromatography, 2012, 30(11): 1194-1202.
[2] Miladys LIMONTA1*, Lourdes ZUMALACRREGUI2, Dayana SOLER1. Adsorption behavior of plasmid DNA onto perfusion chromatographic matrix[J]. Chinese Journal of Chromatography, 2012, 30(05): 522-526.
[3] . [J]. Chinese Journal of Chromatography, 2007, 25(3): 451-452.
[4] . [J]. Chinese Journal of Chromatography, 2005, 23(4): 435-435.
[5] OBATJAZI Seyed Mortaza, SHOJAOSADATI Seyed Abbas, KARBASY Seyed Mojtaba. Purification of L-Lysine in Simulated Moving Bed and Fixed-Bed Chromatography[J]. Chinese Journal of Chromatography, 2004, 22(4): 431-434.
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