Chinese Journal of Chromatography ›› 2018, Vol. 36 ›› Issue (4): 334-338.DOI: 10.3724/SP.J.1123.2017.10007

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Selective enrichment of phosphopeptides with aspartic acid based immobilized metal ion affinity chromatography materials

SHEN Huilian1, ALIMU Kahaer2   

  1. 1. Liaoning National Normal College, Shenyang 110032, China;
    2. Xinjiang Agricultural Vocational Technical College, Changji 831100, China
  • Received:2017-10-20 Online:2018-04-08 Published:2014-02-26

Abstract:

A natural amino acid, aspartic acid, was modified to bind to silica microspheres with a click chemistry method (-Click Asp).The modified Click Asp microspheres were further modified by incorporating Fe3+ ions, resulting in Fe3+ and Click Asp based immobilized ion affinity chromatography (IMAC)(Fe3+-Click Asp).The prepared materials were characterized using Fourier transform infrared spectrometry, X-ray photoelectron spectrometry, and scanning electron microscopy, and it was confirmed that Fe3+-Click Asp was successfully prepared.Subsequently, the Fe3+-Click Asp microspheres were used to enrich phosphopeptides from tryptic digests of proteins and milk.It was found that the phosphopeptides could be selectively enriched with this material.This study describes a novel material and enrichment method for phosphoproteomics.

Key words: aspartic acid, enrichment, immobilized metal ion affinity chromatography (IMAC), phosphopeptide, solid phase extraction (SPE)

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