Progress in separation and purification of nattokinase and its enzyme activity determination

doi:10.3724/SP.J.1123.2018.11010

Abstract

Abstract:

In this paper, the separation and purification of nattokinase have been reviewed by primarily focusing on solvent precipitation, adsorption column chromatography, magnetic microspheres, expanded bed, reverse micelle, and three-phase separation methods. The different methods for determination of the enzyme activity have been discussed and compared. Finally, the feasibility that the nucleic acid-based identification techniques can be used for nattokinase purification and enzyme activity assay has been proposed.

Key words: enzyme activity, nattokinase, review, separation purification

CLC Number:

O658

FA Yun, ZHANG Jinling, ZHAO Haijie, LIU Huizhou. Progress in separation and purification of nattokinase and its enzyme activity determination[J]. Chinese Journal of Chromatography, 2019, 37(3): 274-278.

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