色谱

色谱

• 研究论文 •    下一篇

以亲水作用色谱为核心的液相色谱联用技术及其应用研究

王媛1,2,顾惠新3,路鑫1,许国旺1   

  1. 1.Open Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, the Chinese Academy of Sciences, Dalian 116023, China; 2.Division of Analytical and Environmental Toxicology, Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB, T6G 2G3, Canada; 3.Gansu Langang Petrochemical Co., Ltd., Lanzhou Chemical Company, China National Petroleum Corporation, Lanzhou 730060, China
  • 收稿日期:2008-07-20 修回日期:2008-10-25 出版日期:2008-11-30 发布日期:1983-06-25
  • 通讯作者: 许国旺
  • 基金资助:
    科技部“十一五”国家科技支撑计划(2006BAK02A12)、国家“863”计划(2006AA02Z342)和中国科学院知识创新基金项目

Development of hydrophilic interaction chromatographic hyphenated techniques and their applications

WANG Yuan1,2, GU Huixin3, LU Xin1, XU Guowang1   

  1. 1.Open Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, the Chinese Academy of Sciences, Dalian 116023, China; 2.Division of Analytical and Environmental Toxicology, Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB, T6G 2G3, Canada; 3.Gansu Langang Petrochemical Co., Ltd., Lanzhou Chemical Company, China National Petroleum Corporation, Lanzhou 730060, China
  • Received:2008-07-20 Revised:2008-10-25 Online:2008-11-30 Published:1983-06-25
  • Contact: XU Guowang

PDF

1440

被引次数

107

摘要： 亲水作用色谱(HILIC)是近年来色谱领域研究的热点之一。本文围绕复杂体系样品中亲水性组分的分离分析,综述了国际上近年 来发展的以HILIC为核心的多种液相色谱联用技术及其应用。简要介绍了HILIC的起源、定义、分离特点及其常用固定相;比较了HILIC和反相色谱(RPLC)的选择特性;针对不同层次的分离对象和分离要求,讨论了多种基于HILIC的液相色谱以及液相色谱-质谱联用技术的分离特点和适用范围。

关键词: 多维分离 , 联用技术, 亲水作用色谱, 柱切换

Abstract: Hydrophilic interaction chromatography (HILIC) is popular in the analysis of hydrophilic solutes in recent years. As a unique liquid chromatographic (LC) separation mode, it has not only been successfully utilized for the separation of target compounds from complex matrixes, but also been hyphenated with other separation modes, such as reversed-phase LC (RPLC), ion-exchange chromatography, and various HILIC, to increase the resolution and peak capacity for “entire-component” analysis of complex samples. Here we briefly review the origin and concept of HILIC, its retention behavior, and the frequently used stationary phases. After discussing the selectivity orthogonality between HILIC and RPLC, several application areas of HILIC are introduced, including HILIC tandem mass spectrometry (HILIC-MSn), on-line HILIC pretreatment, comprehensive two-dimensional HILIC-MSn and on-line hyphenation of HILIC and RPLC. With abundant references, HILIC has been proved to be a powerful analytical tool for hydrophilic and polar complexes.

Key words: column-switching, hyphenated techniques, multi-dimensional separation , hydrophilic interaction chromatography (HILIC)