Abstract: A rapid qualitative and quantitative analytical method was developed for the simultaneous determination of 14 heterocyclic aromatic amines (HAAs) in wine by liquid chromatography-ion trap-time of flight tandem mass spectrometry (LC-IT-TOF MS). HAAs were extracted from the samples by ethyl acetate under alkaline condition. The quantitation was carried out using internal standard method. The separation of HAAs was carried out based on Phenomenex Kinetex C18 100A column (100 mm×2.1 mm, 2.6 μm), with a gradient elution of acetonitrile and 30 mmol/L ammonium formate at a flow rate of 0.4 mL/min. The analytes were detected under positive-ion electrospray ionization mode. The results showed that the linear ranges of the 14 HAAs were 1~500 μg/L with limits of detection (signal/noise=3) of 0.33~1.77 μg/L. The average recoveries of all the compounds spiked in wine samples at three levels of 10, 50, 100 μg/L were in the ranges of 71.6%~96.4%, 72.9%~101.9%, 74.5%~103.3%, with the corresponding relative standard deviations (RSDs, n=6) of 2.9%~7.9%、1.7%~5.3%、1.8%~4.8%, respectively. The established method is simple, rapid, accurate, and has wide linear range and high sensitivity. It can be applied to the simultaneous analysis of the HAAs in wine.

Key words: heterocyclic aromatic amines (HAAs), wine, liquid chromatography-ion trap-time of flight tandem mass spectrometry (LC-IT-TOF MS)