Chinese Journal of Chromatography ›› 2017, Vol. 35 ›› Issue (2): 185-190.DOI: 10.3724/SP.J.1123.2016.07008

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Determination of ochratoxin A in wine by direct analysis in real time-tandem mass spectrometry

GONG Xiaoming1, MA Ronghui1, WANG Hongtao1, GUO Liqiang1, LI Kai1, WU Zhenxing2, ZHAO Han1, SUN Jun1   

  1. 1. Weifang Entry-Exit Inspection and Quarantine Bureau, Weifang 261041, China;
    2. Technical Center, Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002, China
  • Received:2016-07-07 Online:2017-02-08 Published:2013-06-09
  • Supported by:

    Scientific Research Fund Project of General Administration of Quality Supervision and Inspection and Quarantine of the Peoples Republic of China (No. 2013IK177).

Abstract:

A method for the determination of ochratoxin A (OTA) in wine was developed using direct analysis in real time-electrospray ionization tandem mass spectrometry (DART-MS/MS). The sample was extracted with ethyl acetate and purified by votexing with ODS powder, detected using DART-MS/MS. The results showed that the average recoveries ranged from 88.7% to 105.7% with the RSDs of 8.5%-12.8% at three spiked levels of 1.0, 2.0, and 10 μ g/kg. The LOQ was 0.5 μg/kg. The proposed method was accurate, rapid and reliable, which can be successfully applied to the qualitative and quantitative analysis of ochratoxin A in wine.

Key words: direct analysis in real time (DART), isotope dilution internal standard, ochratoxin A (OTA), QuEChERS, tandem mass spectrometry (MS/MS), wine

CLC Number: